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In situ hybridization for mRNA detection in Arabidopsis tissue sections

Brewer, Philip B. and Heisler, Marcus G. and Hejátko, Jan and Friml, Jiří and Benková, Eva (2006) In situ hybridization for mRNA detection in Arabidopsis tissue sections. Nature Protocols, 1 (3). pp. 1462-1467. ISSN 1754-2189. https://resolver.caltech.edu/CaltechAUTHORS:20150325-100102764

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Abstract

Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nprot.2006.226 DOIArticle
http://www.nature.com/nprot/journal/v1/n3/full/nprot.2006.226.htmlPublisherArticle
http://rdcu.be/coQ6PublisherFree ReadCube access
ORCID:
AuthorORCID
Heisler, Marcus G.0000-0001-5644-8398
Additional Information:© 2006 Nature Publishing Group. Published online 09 November 2006. We are very grateful to David Smyth for providing laboratory facilities and advice during the establishment of this protocol. J.H. was supported by the Ministry of Education of the Czech Republic (LC06034, MSM0021622415), P.B.B. and J.F. by the VolkswagenStiftung and the EMBO Young Investigator Program, and E.B. by the Margarete von Wrangell-Habilitationsprogramm.
Funders:
Funding AgencyGrant Number
Ministry of Education of the Czech RepublicLC06034
Ministry of Education of the Czech RepublicMSM0021622415
Volkswagen StiftungUNSPECIFIED
European Molecular Biology Organization (EMBO)UNSPECIFIED
Margarete von Wrangell-HabilitationsprogrammUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20150325-100102764
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150325-100102764
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56064
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:25 Mar 2015 19:33
Last Modified:03 Oct 2019 08:10

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