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The homeotic protein AGAMOUS controls microsporogenesis by regulation of SPOROCYTELESS

Ito, Toshiro and Wellmer, Frank and Yu, Hao and Das, Predeep and Ito, Natsuko and Alves-Ferreira, Márcio and Riechmann, José Luis and Meyerowitz, Elliot M. (2004) The homeotic protein AGAMOUS controls microsporogenesis by regulation of SPOROCYTELESS. Nature, 430 (6997). pp. 356-360. ISSN 0028-0836. doi:10.1038/nature02733. https://resolver.caltech.edu/CaltechAUTHORS:20150326-091135183

[img] Image (JPEG) (Supplementary Figure S1 Results of microarray analyses for selected genes) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure S2 Real-time PCR quantitation of SPL/NZZ mRNA accumulation in response to AG-GR activation) - Supplemental Material
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[img] Image (JPEG) (Supplementary Figure S3 The CArG box-like sequence in the 3' region of SPL/NZZ is required for its normal expression in the integuments and the funiculus of developing ovules) - Supplemental Material
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[img] MS Word (Supplementary Table 1 Primer and oligonucleotide sequences) - Supplemental Material
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Abstract

The Arabidopsis homeotic gene AGAMOUS (AG) is necessary for the specification of reproductive organs (stamens and carpels) during the early steps of flower development. AG encodes a transcription factor of the MADS-box family that is expressed in stamen and carpel primordia. At later stages of development, AG is expressed in distinct regions of the reproductive organs. This suggests that AG might function during the maturation of stamens and carpels, as well as in their early development. However, the developmental processes that AG might control during organogenesis and the genes that are regulated by this factor are largely unknown. Here we show that microsporogenesis, the process leading to pollen formation, is induced by AG through activation of the SPOROCYTELESS gene (SPL, also known as NOZZLE,NZZ), a regulator of sporogenesis. Furthermore, we demonstrate that SPL can induce microsporogenesis in the absence of AG function, suggesting that AG controls a specific process during organogenesis by activating another regulator that performs a subset of its functions.


Item Type:Article
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URLURL TypeDescription
http://dx.doi.org/10.1038/nature02733DOIArticle
http://www.nature.com/nature/journal/v430/n6997/full/nature02733.htmlPublisherArticle
http://www.nature.com/nature/journal/v430/n6997/suppinfo/nature02733.htmlPublisherSupplementary Information
http://rdcu.be/cpqpPublisherFree ReadCube access
http://www.nature.com/nature/journal/v430/n6997/extref/nature02733-s7.htmPublisherSupplementary Data This includes an HTML Contents Table linking to the microarray time course, processed data and raw data files
ORCID:
AuthorORCID
Ito, Toshiro0000-0002-8206-2787
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2004 Nature Publishing Group. Received 9 May; accepted 7 June 2004. We thank J. Chow for experimental help; M. Hasebe; former Meyerowitz laboratory members, J. A. Long, K. Goto, M. Frohlich and P. Kumar; and current laboratory members, especially P. Sieber, I. Negrutiu, A. Dubois, C. Ohno, C. Baker, E. Haswell, M. Heisler, V. R. Gonehal and Y. X. Zhao for comments on the manuscript. M.A-F. is indebted to IPBO and M. Van Montagu for a fellowship sponsored by Aventis CropSciences. This work was supported by a grant from the National Institutes of Health to E.M.M.
Funders:
Funding AgencyGrant Number
Aventis CropSciencesUNSPECIFIED
NIHUNSPECIFIED
Issue or Number:6997
DOI:10.1038/nature02733
Record Number:CaltechAUTHORS:20150326-091135183
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150326-091135183
Official Citation:Ito, T., Wellmer, F., Yu, H., Das, P., Ito, N., Alves-Ferreira, M., . . . Meyerowitz, E. M. (2004). The homeotic protein AGAMOUS controls microsporogenesis by regulation of SPOROCYTELESS. [10.1038/nature02733]. Nature, 430(6997), 356-360.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56112
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:26 Mar 2015 20:17
Last Modified:10 Nov 2021 20:54

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