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In Vivo Synthesis and Structural Analysis of Alanylglycine-Rich Artificial Proteins

Cantor, Eric J. and Creel, Howard S. and Deguchi, Yoshikuni and Dougherty, Michael J. and Kothakota, Srinivas and Krejchi, Mark T. and Matsuki, Kunio and McGrath, Kevin P. and Parkhe, Ajay D. and Atkins, Edward D. T. and Fournier, Maurille J. and Mason, Thomas L. and Tirrell, David A. (1994) In Vivo Synthesis and Structural Analysis of Alanylglycine-Rich Artificial Proteins. In: Silk Polymers: Materials Science and Biotechnology. ACS Symposium Series. No.55. American Chemical Society , Washington, DC, pp. 98-103. ISBN 9780841227439. https://resolver.caltech.edu/CaltechAUTHORS:20150326-115039794

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Abstract

A series of 22 alanylglycine-rich artificial proteins has been prepared by bacterial expression of the corresponding synthetic genes. These polymers have been isolated and purified, and subjected to structural analysis by gel electrophoresis, mass spectrometry, x-ray scattering, and vibrational and NMR spectroscopy. Strategies have been developed for the in vivo synthesis of artificial proteins containing non-natural amino acids.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bk-1994-0544.ch009DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bk-1994-0544.ch009PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 1994 American Chemical Society. Received May 4, 1993.
Series Name:ACS Symposium Series
Issue or Number:55
Record Number:CaltechAUTHORS:20150326-115039794
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150326-115039794
Official Citation:In Vivo Synthesis and Structural Analysis of Alanylglycine-Rich Artificial Proteins Eric J. Cantor, Howard S. Creel, Yoshikuni Deguchi, Michael J. Dougherty, Srinivas Kothakota, Mark T. Krejchi, Kunio Matsuki, Kevin P. McGrath, Ajay D. Parkhe, Edward D. T. Atkins, Maurille J. Fournier, Thomas L. Mason, and David A. Tirrell Silk Polymers. December 3, 1993, 98-103 Doi:10.1021/bk-1994-0544.ch009
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56139
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:27 Mar 2015 01:57
Last Modified:03 Oct 2019 08:11

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