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Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1

Chen, Ching-Kang and Burns, Marie E. and He, Wei and Wensel, Theodore G. and Baylor, Denis A. and Simon, Melvin I. (2000) Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1. Nature, 403 (6769). pp. 557-560. ISSN 0028-0836. http://resolver.caltech.edu/CaltechAUTHORS:20150330-093839881

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Abstract

Timely deactivation of the ɑ-subunit of the rod G-protein transducin (Gɑt) is essential for the temporal resolution of rod vision. Regulators of G-protein signalling (RGS) proteins accelerate hydrolysis of GTP by the ɑ-subunits of heterotrimeric G proteins in vitro. Several retinal RGS proteins can act in vitro as GTPase accelerating proteins (GAP) for Gɑt. Recent reconstitution experiments indicate that one of these, RGS9-1, may account for much of the Gɑt GAP activity in rod outer segments (ROS). Here we report that ROS membranes from mice lacking RGS9-1 hydrolyse GTP more slowly than ROS membranes from control mice. The Gβ5-L protein that forms a complex with RGS9-1 (ref. 10) was absent from RGS9-/- retinas, although Gβ5-L messenger RNA was still present. The flash responses of RGS9-/- rods rose normally, but recovered much more slowly than normal. We conclude that RGS9-1, probably in a complex with Gβ5-L, is essential for acceleration of hydrolysis of GTP by Gɑt and for normal recovery of the photoresponse.


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http://dx.doi.org/10.1038/35000601DOIArticle
http://www.nature.com/nature/journal/v403/n6769/abs/403557a0.htmlPublisherArticle
http://rdcu.be/cq1FPublisherFree ReadCube access
Additional Information:© 2000 Macmillan Magazines Ltd. Received 5 October 1999; Accepted 24 November 1999. We thank members of the Caltech Transgenic Core Facility for their technical support, and R. Lefkowitz, J. Hurley and H. Jurgen-Fuller for antibodies. This work was supported by grants from the NIH.
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Record Number:CaltechAUTHORS:20150330-093839881
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150330-093839881
Official Citation:Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1 p557 Ching-Kang Chen, Marie E. Burns, Wei He, Theodore G. Wensel, Denis A. Baylor and Melvin I. Simon doi:10.1038/35000601
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ID Code:56207
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:30 Mar 2015 20:07
Last Modified:30 Mar 2015 20:07

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