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Dishevelled controls cell polarity during Xenopus gastrulation

Wallingford, John B. and Rowning, Brian A. and Vogeli, Kevin M. and Rothbächer, Ute and Fraser, Scott E. and Harland, Richard M. (2000) Dishevelled controls cell polarity during Xenopus gastrulation. Nature, 405 (6782). pp. 81-85. ISSN 0028-0836. https://resolver.caltech.edu/CaltechAUTHORS:20150330-103933909

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Abstract

Although cell movements are vital for establishing the normal architecture of embryos, it is unclear how these movements are regulated during development in vertebrates. Inhibition of Xenopus Dishevelled (Xdsh) function disrupts convergent extension movements of cells during gastrulation, but the mechanism of this effect is unclear, as cell fates are not affected. In Drosophila, Dishevelled controls both cell fate and cell polarity but whether Dishevelled is involved in controlling cell polarity in vertebrate embryos has not been investigated. Here we show, using time-lapse confocal microscopy, that the failure of cells lacking Xdsh function to undergo convergent extension results from defects in cell polarity. Furthermore, Xdsh mutations that inhibit convergent extension correspond to mutations in Drosophila Dishevelled that selectively perturb planar cell polarity. Finally, the localization of Xdsh at the membrane of normal dorsal mesodermal cells is consistent with Xdsh controlling cell polarity. Our results show that polarized cell behaviour is essential for convergent extension and is controlled by vertebrate Dishevelled. Thus, a vertebrate equivalent of the Drosophila planar cell polarity signalling cascade may be required for normal gastrulation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/35011077DOIArticle
http://www.nature.com/nature/journal/v405/n6782/abs/405081a0.htmlPublisherArticle
http://rdcu.be/cq1vPublisherFree ReadCube access
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2000 Macmillan Magazines Ltd. Received 13 January 2000; Accepted 24 March 2000. We thank T. Grammer, M. Dionne, A. Sater, D. Keys, K. Liu and D. Frank for discussions, reading the manuscript, and blind scoring; S. Sokol for the Xdsh and Xdd1 plasmids; and D. Turner for the memEGFP plasmid. This work was supported by the NIH, NIMH and the Beckman Institute. J.B.W. was supported by NIH/NIGMS and ACS postdoctoral fellowships; B.A.R. by a US Department of Defense Breast Cancer Research Program Grant to C. Larabell; and K.M.V. by the Nathan and Violet David Scholars program at UC Berkeley.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
National Institute of Mental Health (NIMH) UNSPECIFIED
Caltech Beckman Institute UNSPECIFIED
National Institute of General Medical SciencesUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Department of Defense Breast Cancer Research ProgramUNSPECIFIED
University of California, BerkeleyUNSPECIFIED
Issue or Number:6782
Record Number:CaltechAUTHORS:20150330-103933909
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150330-103933909
Official Citation:Dishevelled controls cell polarity during Xenopus gastrulation p81 John B. Wallingford, Brian A. Rowning, Kevin M. Vogeli, Ute Rothbächer, Scott E. Fraser and Richard M. Harland doi:10.1038/35011077
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56212
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:30 Mar 2015 19:53
Last Modified:03 Oct 2019 08:12

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