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Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA

Pasquinelli, Amy E. and Reinhart, Brenda J. and Slack, Frank and Martindale, Mark Q. and Kuroda, Mitzi I. and Maller, Betsy and Hayward, David C. and Ball, Eldon E. and Degnan, Bernard and Müller, Peter and Spring, Jürg and Srinivasan, Ashok and Fishman, Mark and Finnerty, John and Corbo, Joseph and Levine, Michael and Leahy, Patrick and Davidson, Eric and Ruvkun, Gary (2000) Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature, 408 (6808). p. 86. ISSN 0028-0836. doi:10.1038/35040556.

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Two small RNAs regulate the timing of Caenorhabditis elegans development. Transition from the first to the second larval stage fates requires the 22-nucleotide lin-4 RNA and transition from late larval to adult cell fates requires the 21-nucleotide let-7 RNA. The lin-4 and let-7 RNA genes are not homologous to each other, but are each complementary to sequences in the 3' untranslated regions of a set of protein-coding target genes that are normally negatively regulated by the RNAs. Here we have detected let-7 RNAs of ~21 nucleotides in samples from a wide range of animal species, including vertebrate, ascidian, hemichordate, mollusc, annelid and arthropod, but not in RNAs from several cnidarian and poriferan species, Saccharomyces cerevisiae, Escherichia coli or Arabidopsis. We did not detect lin-4 RNA in these species. We found that let-7 temporal regulation is also conserved: let-7 RNA expression is first detected at late larval stages in C. elegans and Drosophila , at 48 hours after fertilization in zebrafish, and in adult stages of annelids and molluscs. The let-7 regulatory RNA may control late temporal transitions during development across animal phylogeny.

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Pasquinelli, Amy E.0000-0002-9511-0039
Müller, Peter0000-0002-0702-6209
Additional Information:© 2000 Macmillan Magazines Ltd. Received 12 June 2000; Accepted 11 August 2000. We thank the following people for RNA and tissue samples: T. Heanue, R. Pearse and C. Tabin for chick; J. Gerhart and M. Kirschner for Xenopus and acorn worm; S. Agarwal for Xenopus; N. Stavropoulos for mouse; C. Unabia and K. del Carmen for annelid and mollusc; H. Bode for Hydra; J. Nardone and S. Ferrari for Arabidopsis; P. Sudarsanam for yeast; and D. Selinger for E. coli. The phylogenetic survey in this work was inspired by the NASA Evolution and Development meetings organized by E. Davidson and C. Golden. This work was supported by an NIH grant from NIGMS to G.R. and a grant from the MGH Fund for Medical Discovery to A.E.P.
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Massachusetts General HospitalUNSPECIFIED
Issue or Number:6808
Record Number:CaltechAUTHORS:20150330-111356018
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Official Citation:Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA p86 Amy E. Pasquinelli, Brenda J. Reinhart, Frank Slack, Mark Q. Martindale, Mitzi I. Kuroda, Betsy Maller, David C. Hayward, Eldon E. Ball, Bernard Degnan, Peter Müller, Jürg Spring, Ashok Srinivasan, Mark Fishman, John Finnerty, Joseph Corbo, Michael Levine, Patrick Leahy, Eric Davidson and Gary Ruvkun doi:10.1038/35040556
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56216
Deposited By: Ruth Sustaita
Deposited On:30 Mar 2015 19:41
Last Modified:10 Nov 2021 20:56

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