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Specification of the neural crest occurs during gastrulation and requires Pax7

Basch, Martín L. and Bronner-Fraser, Marianne and García-Castro, Martin I. (2006) Specification of the neural crest occurs during gastrulation and requires Pax7. Nature, 441 (7090). pp. 218-222. ISSN 0028-0836. https://resolver.caltech.edu/CaltechAUTHORS:20150331-151012740

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Abstract

The neural crest is a stem population critical for development of the vertebrate craniofacial skeleton and peripheral ganglia. Neural crest cells originate along the border between the neural plate and epidermis, migrate extensively and generate numerous derivatives, including neurons and glia of the peripheral nervous system, melanocytes, bone and cartilage of the head skeleton. Impaired neural crest development is associated with human defects, including cleft palate. Classically, the neural crest has been thought to form by interactions at the border between neural and non-neural ectoderm or mesoderm, and defined factors such as bone morphogenetic proteins (BMPs) and Wnt proteins have been postulated as neural crest-inducers. Although competence to induce crest cells declines after stage 10 (ref. 14), little is known about when neural crest induction begins in vivo. Here we report that neural crest induction is underway during gastrulation and well before proper neural plate appearance. We show that a restricted region of chick epiblast (stage 3–4) is specified to generate neural crest cells when explanted under non-inducing conditions. This region expresses the transcription factor Pax7 by stage 4 + and later contributes to neural folds and migrating neural crest. In chicken embryos, Pax7 is required for neural crest formation in vivo, because blocking its translation inhibits expression of the neural crest markers Slug, Sox9, Sox10 and HNK-1. Our results indicate that neural crest specification initiates earlier than previously assumed, independently of mesodermal and neural tissues, and that Pax7 has a crucial function during neural crest development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nature04684DOIArticle
http://www.nature.com/nature/journal/v441/n7090/full/nature04684.htmlPublisherArticle
http://www.nature.com/nature/journal/v441/n7090/suppinfo/nature04684.htmlPublisherSupporting Information
http://rdcu.be/cryPPublisherFree Readcube access
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2006 Nature Publishing Group. Received 10 November 2005; Accepted 23 February 2006. We thank A. Streit, S. Fraser, A. Groves, L. Gammill, V. Lee, J. Sechrist and T. Moreno for comments and discussions; S. Mackem, D. Wilkinson, E. Matsunaga, A. Kawakami, O. Pourquie´ and H. Kondoh for gifts of Tbx6l, cMsx1, full-length cPax3, full-length cPax7, BMP4, Sox2, Dlx5 and Gata2 probes and constructs; and D. Arce and Y. Liu for technical assistance. This work was supported by a USPHS grant to M.B.-F.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)UNSPECIFIED
Issue or Number:7090
Record Number:CaltechAUTHORS:20150331-151012740
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150331-151012740
Official Citation:Basch, M. L., Bronner-Fraser, M., & García-Castro, M. I. (2006). Specification of the neural crest occurs during gastrulation and requires Pax7. [10.1038/nature04684]. Nature, 441(7090), 218-222.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56258
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:31 Mar 2015 23:15
Last Modified:03 Oct 2019 08:12

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