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A nanoliter-scale nucleic acid processor with parallel architecture

Hong, Jong Wook and Studer, Vincent and Hang, Giao and Anderson, W. French and Quake, Stephen R. (2004) A nanoliter-scale nucleic acid processor with parallel architecture. Nature Biotechnology, 22 (4). pp. 435-439. ISSN 1087-0156.

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The purification of nucleic acids from microbial and mammalian cells is a crucial step in many biological and medical applications1. We have developed microfluidic chips for automated nucleic acid purification from small numbers of bacterial or mammalian cells. All processes, such as cell isolation, cell lysis, DNA or mRNA purification, and recovery, were carried out on a single microfluidic chip in nanoliter volumes without any pre- or postsample treatment. Measurable amounts of mRNA were extracted in an automated fashion from as little as a single mammalian cell and recovered from the chip. These microfluidic chips are capable of processing different samples in parallel, thereby illustrating how highly parallel microfluidic architectures can be constructed to perform integrated batch-processing functionalities for biological and medical applications.

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Additional Information:© 2004 Nature Publishing Group. The authors would like to thank Kathy Burke for assistance with the RT-PCR and Carl Hansen and James P. Landers for helpful discussions. This work was supported in part by the National Science Foundation (NSF), the Department of Defense Advanced Research Projects Agency (DARPA) and a gift from Farmal Biomedicine, LLC.
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Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Farmal Biomedicine, LLCUNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20150401-130206631
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56292
Deposited By: Tony Diaz
Deposited On:01 Apr 2015 20:34
Last Modified:03 Oct 2019 08:12

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