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Planar cell polarity signalling controls cell division orientation during zebrafish gastrulation

Gong, Ying and Mo, Chunhui and Fraser, Scott E. (2004) Planar cell polarity signalling controls cell division orientation during zebrafish gastrulation. Nature, 430 (7000). pp. 689-693. ISSN 0028-0836. doi:10.1038/nature02796.

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Oriented cell division is an integral part of pattern development in processes ranging from asymmetric segregation of cell-fate determinants to the shaping of tissues. Despite proposals that it has an important function in tissue elongation, the mechanisms regulating division orientation have been little studied outside of the invertebrates Caenorhabditis elegans and Drosophila melanogaster1. Here, we have analysed mitotic divisions during zebrafish gastrulation using in vivo confocal imaging and found that cells in dorsal tissues preferentially divide along the animal–vegetal axis of the embryo. Establishment of this animal–vegetal polarity requires the Wnt pathway components Silberblick/Wnt11, Dishevelled and Strabismus. Our findings demonstrate an important role for non-canonical Wnt signalling in oriented cell division during zebrafish gastrulation, and indicate that oriented cell division is a driving force for axis elongation. Furthermore, we propose that non-canonical Wnt signalling has a conserved role in vertebrate axis elongation, orienting both cell intercalation and mitotic division.

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Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2004 Nature Publishing Group. Received 7 May 2004; Accepted 30 June 2004; Published online 14 July 2004. We thank M. Hibi (Zdkk1), M. Park (Stbm MO), J. Wallingford (Xdsh-D2), R. Köster (H2B–GFP), S. Megason (H2B–RFP1 and membrane RFP1), M. Tada (Xdsh-DPDZ and Xdsh-DEP +) and H. McBride (ICAT) for providing the plasmids and morpholino, and C.-P. Heisenberg for the silberblick/wnt11 mutants. We also thank S. Bhattacharyya, M. Bronner-Fraser, M. Garcia-Castro, D. Koos, R. Köster, B. Link, S. Megason and J. Wallingford for discussion and critical reading of the manuscript.
Issue or Number:7000
Record Number:CaltechAUTHORS:20150401-134153962
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56294
Deposited By: Tony Diaz
Deposited On:01 Apr 2015 21:21
Last Modified:10 Nov 2021 20:57

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