A Caltech Library Service

Visualization of membrane protein domains by cryo-electron microscopy of dengue virus

Zhang, Wei and Chipman, Paul R. and Corver, Jeroen and Johnson, Peter R. and Zhang, Ying and Mukhopadhyay, Suchetana and Baker, Timothy S. and Strauss, James H. and Rossmann, Michael G. and Kuhn, Richard J. (2003) Visualization of membrane protein domains by cryo-electron microscopy of dengue virus. Nature Structural Biology, 10 (11). pp. 907-912. ISSN 1072-8368. PMCID PMC4148076.

[img] PDF - Accepted Version
See Usage Policy.


Use this Persistent URL to link to this item:


Improved technology for reconstructing cryo-electron microscopy (cryo-EM) images has now made it possible to determine secondary structural features of membrane proteins in enveloped viruses. The structure of mature dengue virus particles was determined to a resolution of 9.5 Å by cryo-EM and image reconstruction techniques, establishing the secondary structural disposition of the 180 envelope (E) and 180 membrane (M) proteins in the lipid envelope. The ɑ-helical 'stem' regions of the E molecules, as well as part of the N-terminal section of the M proteins, are buried in the outer leaflet of the viral membrane. The 'anchor' regions of E and the M proteins each form antiparallel E-E and M-M transmembrane alpha-helices, leaving their C termini on the exterior of the viral membrane, consistent with the predicted topology of the unprocessed polyprotein. This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Additional Information:© 2003 Nature Publishing Group. Received 16 May 2003; Accepted 30 July 2003; Published online: 5 October 2003. We thank E. Strauss for helpful discussions and R. Ashmore, C.R. Xiao, Y. Ji and D. Marinescu for the use of their various computer programs that were essential for calculating the image reconstruction. We are grateful for an equipment grant from the Keck Foundation. This work was supported by a US National Institutes of Health (NIH) Program Project grant to M.G.R., R.J.K. and T.S.B., by NIH grants to T.S.B. and J.H.S. and by a US National Science Foundation grant to T.S.B.
Funding AgencyGrant Number
W. M. Keck FoundationUNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC4148076
Record Number:CaltechAUTHORS:20150407-134213554
Persistent URL:
Official Citation:Visualization of membrane protein domains by cryo-electron microscopy of dengue virus pp907 - 912 Wei Zhang, Paul R Chipman, Jeroen Corver, Peter R Johnson, Ying Zhang, Suchetana Mukhopadhyay, Timothy S Baker, James H Strauss, Michael G Rossmann & Richard J Kuhn Published online: 05 October 2003 | doi:10.1038/nsb990
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56439
Deposited By: Ruth Sustaita
Deposited On:07 Apr 2015 21:16
Last Modified:03 Oct 2019 08:13

Repository Staff Only: item control page