CaltechAUTHORS
  A Caltech Library Service

Global Analysis of Protein Phosphorylation in Yeast

Ptacek, Jason and Mah, Angie S. (2006) Global Analysis of Protein Phosphorylation in Yeast. FASEB Journal, 20 (Suppl). A1308. ISSN 0892-6638. https://resolver.caltech.edu/CaltechAUTHORS:20150407-141244835

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20150407-141244835

Abstract

Protein phosphorylation is estimated to affect 30% of the proteome and is a major regulatory mechanism that controls many basic cellular processes. Until recently, our biochemical understanding of protein phosphorylation on a global scale has been extremely limited; only one half of the yeast kinases have known in vivo substrates and for less than 160 phosphoproteins is the phosphorylating kinase known. Using proteome chip technology we determined the in vitro substrates recognized by the majority of yeast protein kinases. Over 4000 phosphorylation events involving 1,325 different proteins were identified; these substrates represent a broad spectrum of different biochemical functions and cellular roles. Distinct sets of substrates were recognized by each protein kinase, including closely related kinases of the protein kinase A family and four CDKs that vary only in their cyclin subunits. While many substrates reside in the same cellular compartment or belong to the same functional category as their phosphorylating kinase, many others do not, suggesting new roles for a number of kinases. Furthermore, integration of the phosphorylation results with protein-protein interaction and transcription factor binding data revealed a number of novel regulatory modules. Our phosphorylation results have been assembled into a first generation phosphorylation map for yeast. Since many yeast proteins and pathways are conserved, these results provide insights into the mechanisms and roles of protein phosphorylation in many eukaryotes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.fasebj.org/cgi/content/meeting_abstract/20/5/A1308?sid=c68c25d5-a620-4009-a653-6ee0fff760d7PublisherArticle
Additional Information:© 2006 FASEB.
Issue or Number:Suppl
Record Number:CaltechAUTHORS:20150407-141244835
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150407-141244835
Official Citation:Jason Ptacek, Geeta Devgan, Gregory Michaud, Heng Zhu, Xiaowei Zhu, Joseph Fasolo, Hong Guo, Ghil Jona, Ashton Breitkreutz, Richelle Sopko, Rhonda R. McCartney, Martin C. Schmidt, Najma Rachidi, Soo-Jung Lee, Angie S. Mah, Lihao Meng, Michael J.R. Stark, David F. Stern, Claudio De Virgilio, Mike Tyers, Brenda Andrews, Mark Gerstein, Barry Schweitzer, Paul F. Predki, and Michael Snyder Global Analysis of Protein Phosphorylation in Yeast FASEB J October 28, 2006 20:A1308
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56441
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:07 Apr 2015 21:36
Last Modified:03 Oct 2019 08:13

Repository Staff Only: item control page