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Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension

Schense, Jason C. and Bloch, Jocelyne and Aebischer, Patrick and Hubell, Jeffrey A. (2000) Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension. Nature Biotechnology, 18 (4). pp. 415-419. ISSN 1087-0156. http://resolver.caltech.edu/CaltechAUTHORS:20150407-150724398

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Abstract

Fibrin plays an important role in wound healing and regeneration, and enjoys widespread use in surgery and tissue engineering. The enzymatic activity of Factor XIIIa was employed to covalently incorporate exogenous bioactive peptides within fibrin during coagulation. Fibrin gels were formed with incorporated peptides from laminin and N-cadherin alone and in combination at concentrations up to 8.2 mol peptide per mole of fibrinogen. Neurite extension in vitro was enhanced when gels were augmented with exogenous peptide, with the maximal improvement reaching 75%. When this particular fibrin derivative was evaluated in rats in the repair of the severed dorsal root within polymeric tubes, the number of regenerated axons was enhanced by 85% relative to animals treated with tubes filled with unmodified fibrin. These results demonstrate that it is possible to enhance the biological activity of fibrin by enzymatically incorporating exogenous oligopeptide domains of morphoregulatory proteins.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/74473DOIArticle
http://www.nature.com/nbt/journal/v18/n4/abs/nbt0400_415.htmlPublisherArticle
Additional Information:© 2000 Nature America Inc. Received 19 November 1999; accepted14 January 2000. This work was funded by Grant 31-52261 NFP 38 of the Swiss National Science Foundation.
Funders:
Funding AgencyGrant Number
Swiss National Science Foundation31-52261 NFP 38
Subject Keywords:Fibrin, factor XIII, nerve regeneration, laminin, N-cadherin
Record Number:CaltechAUTHORS:20150407-150724398
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150407-150724398
Official Citation:Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension - pp415 - 419 Jason C. Schense, Jocelyne Bloch, Patrick Aebischer & Jeffrey A. Hubbell doi:10.1038/74473
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56445
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:07 Apr 2015 22:22
Last Modified:07 Apr 2015 22:22

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