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Eph Family Transmembrane Ligands Can Mediate Repulsive Guidance of Trunk Neural Crest Migration and Motor Axon Outgrowth

Wang, Hai U. and Anderson, David J. (1997) Eph Family Transmembrane Ligands Can Mediate Repulsive Guidance of Trunk Neural Crest Migration and Motor Axon Outgrowth. Neuron, 18 (3). pp. 383-396. ISSN 0896-6273. https://resolver.caltech.edu/CaltechAUTHORS:20150408-071323625

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Abstract

In vertebrate embryos, neural crest cell migration and motor axon outgrowth are restricted to rostral somite halves by repulsive factors located in the caudal somite compartment. We show that two Eph family transmembrane ligands, Lerk2 and HtkL, are expressed in caudal somite halves, and that crest cells and motor axons express receptors for these ligands. In several independent in vitro assays, preclustered ligand-Fc fusion proteins can repulsively guide both crest migration and motor axon outgrowth. These repulsive activities depend on a graded or discontinuous presentation of the ligands when tested in the context of permissive substrates, such as laminin or fibronectin. These results identify Lerk2 and HtkL as potential determinants of segmental pattern in the peripheral nervous system.


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http://dx.doi.org/10.1016/S0896-6273(00)81240-4 DOIArticle
ORCID:
AuthorORCID
Anderson, David J.0000-0001-6175-3872
Additional Information:© 1997 by Cell Press. Under an Elsevier user license. Received December 27, 1996; revised January 24, 1997. We thank A. Groves and N. Shah for teaching the in situ hybridization and neural crest explant procedures, respectively; S. Fashena for the hIgG1-Fc vector; L. Lo for anti-c-ret antibody; T. Frankfurter for TUJ1 antibody; J. Lauder and J. Moiseiwitsch for advice on the chemotaxis assay; J. Vielmetter and C. Krull for advice on the stripe assay, and C. Marcelle for help in the interpretation of the chick in situs. We are particularly grateful to F. Bonhoeffer for providing silicone matrices for use in the stripe assays. We thank N. Gale and G. Yancopoulos for sharing unpublished results, and S. Kulkarni, T. Pawson, and R. Klein for Nuk/Sek4 mutant embryos. We also thank D. Wilkinson, S. Fraser, and M. Bronner-Fraser for communicating their unpublished results and for comments on the manuscript, and our lab members for discussions and support. D. J. A. is an Investigator of the Howard Hughes Medical Institute.
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Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20150408-071323625
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150408-071323625
Official Citation:Hai U. Wang, David J. Anderson, Eph Family Transmembrane Ligands Can Mediate Repulsive Guidance of Trunk Neural Crest Migration and Motor Axon Outgrowth, Neuron, Volume 18, Issue 3, March 1997, Pages 383-396, ISSN 0896-6273, http://dx.doi.org/10.1016/S0896-6273(00)81240-4. (http://www.sciencedirect.com/science/article/pii/S0896627300812404)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56453
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Apr 2015 23:06
Last Modified:27 Mar 2020 23:08

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