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Label-free electrochemical detection of human methyltransferase from tumors

Furst, Ariel L. and Muren, Natalie and Hill, Michael G. and Barton, Jacqueline K. (2015) Label-free electrochemical detection of human methyltransferase from tumors. Abstracts of Papers of the American Chemical Society, 249 . INOR-929. ISSN 0065-7727. https://resolver.caltech.edu/CaltechAUTHORS:20150408-095933576

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Abstract

Abnormal DNA methyltransferase activity has been implicated in cancerous transformations and tumorigenesis; efficient detection of methyltransferase activity could provide an early cancer diagnostic. Currently, most detection platforms involve fluorescence or radioactivity, making them cumbersome. Electrochem. platforms, in contrast, offer high portability, sensitivity, and ease of use. We have developed an electrochem. DNA array platform that is formed and addressed using a secondary electrode, allowing for both formation of dil. DNA monolayers and optimized detection through DNA-mediated charge transport with electrocatalysis. This label-free electrochem. platform has been used to successfully detect the activity of the most abundant human methyltransferase, DNMT1, in crude lysates from both cultured human colorectal cancer cells (HCT116) and colorectal tissue samples. We addnl. distinguish colorectal carcinoma tissue from healthy adjacent tissue through differences in DNMT1 activity, demonstrating the utility of this two-electrode platform for clin. applications.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.acs.org/content/acs/en/meetings/spring-2015.htmlOrganizationConference Website
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2015 American Chemical Society.
Record Number:CaltechAUTHORS:20150408-095933576
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150408-095933576
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56474
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Apr 2015 20:46
Last Modified:03 Oct 2019 08:14

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