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Single-site enzymatic cleavage of yeast genomic DNA mediated by triple helix formation

Strobel, Scott A. and Dervan, Peter B. (1991) Single-site enzymatic cleavage of yeast genomic DNA mediated by triple helix formation. Nature, 350 (6314). pp. 172-174. ISSN 0028-0836. https://resolver.caltech.edu/CaltechAUTHORS:20150409-160724386

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Abstract

Physical mapping of chromosomes would be facilitated by methods of breaking large DNA into manageable fragments, or cutting uniquely at genetic markers of interest. Key issues in the design of sequence-specific DNA cleaving reagents are the specificity of binding, the generalizability of the recognition motif, and the cleavage yield. Oligonucleotide-directed triple helix formation is a generalizable motif for specific binding to sequences longer than 12 base pairs within DNA of high complexity. Studies with plasmid DNA show that triple helix formation can limit the operational specificity of restriction enzymes to endonu-clease recognition sequences that overlap oligonucleotide-binding sites. Triple helix formation, followed by methylase protection, triple helix-disruption, and restriction endonuclease digestion produces near quantitative cleavage at the single overlapping triple helix–endonuclease site. As a demonstration that this technique may be applicable to the orchestrated cleavage of large genomic DNA, we report the near quantitative single-site enzymatic cleavage of the Saccharomyces cerevisiae genome mediated by triple helix formation. The 340-kilobase yeast chromosome HI was cut uniquely at an overlapping homopurine–Ec0RI target site 27 base pairs long to produce two expected cleavage products of 110 and 230 kilobases. No cleavage of any other chromosome was detected. The potential generalizability of this technique, which is capable of near quantitative cleavage at a single site in at least 14 megabase pairs of DNA, could enable selected regions of chromosomal DNA to be isolated without extensive screening of genomic libraries.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/350172a0 DOIArticle
http://www.nature.com/nature/journal/v350/n6314/abs/350172a0.htmlPublisherArticle
Additional Information:© 1991 Nature Publishing Group. Received 5 October: accepted 20 December 1990. We thank J. Hanish for helpful discussions. The work was supported by the National Institutes of Health and the Howard Hughes Medical Institute (predoctoral fellowship to S.A.S.).
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Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:6314
Record Number:CaltechAUTHORS:20150409-160724386
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150409-160724386
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56561
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Apr 2015 00:45
Last Modified:03 Oct 2019 08:15

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