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Optofluidic ultrahigh-throughput detection of fluorescent drops

Kim, Minkyu and Pan, Ming and Gai, Ya and Pang, Shuo and Han, Chao and Yang, Changhuei and Tang, Sindy K. Y. (2015) Optofluidic ultrahigh-throughput detection of fluorescent drops. Lab on a Chip, 15 (6). pp. 1417-1423. ISSN 1473-0197. https://resolver.caltech.edu/CaltechAUTHORS:20150410-093758340

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Abstract

This paper describes an optofluidic droplet interrogation device capable of counting fluorescent drops at a throughput of 254000 drops per second. To our knowledge, this rate is the highest interrogation rate published thus far. Our device consists of 16 parallel microfluidic channels bonded directly to a filter-coated two-dimensional Complementary Metal-Oxide-Semiconductor (CMOS) sensor array. Fluorescence signals emitted from the drops are collected by the sensor that forms the bottom of the channel. The proximity of the drops to the sensor facilitates efficient collection of fluorescence emission from the drops, and overcomes the trade-off between light collection efficiency and field of view in conventional microscopy. The interrogation rate of our device is currently limited by the acquisition speed of CMOS sensor, and is expected to increase further as high-speed sensors become increasingly available.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/c4lc01465kDOIArticle
http://pubs.rsc.org/en/Content/ArticleLanding/2015/LC/C4LC01465KPublisherArticle
http://www.rsc.org/suppdata/lc/c4/c4lc01465k/c4lc01465k1.pdfPublisherSupplementary information
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2015 The Royal Society of Chemistry. Received 15th December 2014, Accepted 8th January 2015, First published online 08 Jan 2015. We acknowledge Fengjiao Lyu for helpful discussions and assistance with initial experiments. We also acknowledge partial support from the California Sea Grant College Program (CASG), the Stanford Woods Institute for the Environment, Stanford Nano Shared Facilities Bio/Medical Mini Seed Grant, and the Stanford Center for Innovation in Global Health. ST acknowledges additional support from the 3M Non-tenured Faculty Award.
Funders:
Funding AgencyGrant Number
California Sea Grant College ProgramUNSPECIFIED
Stanford Woods Institute for the EnvironmentUNSPECIFIED
Stanford Nano Shared Facilities Bio/Medical Mini Seed GrantUNSPECIFIED
Stanford Center for Innovation in Global HealthUNSPECIFIED
3M CorporationUNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20150410-093758340
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150410-093758340
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56569
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Apr 2015 17:33
Last Modified:09 Mar 2020 13:19

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