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Neuronal P2X transmitter-gated cation channels change their ion selectivity in seconds

Khakh, Baljit S. and Bao, Xiaoyan R. and Labarca, Cesar and Lester, Henry A. (1999) Neuronal P2X transmitter-gated cation channels change their ion selectivity in seconds. Nature Neuroscience, 2 (4). pp. 322-330. ISSN 1097-6256. doi:10.1038/7233.

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Fast synaptic transmission depends on the selective ionic permeability of transmitter-gated ion channels. Here we show changes in the ion selectivity of neuronal P2X transmitter-gated cation channels as a function of time (on the order of seconds) and previous ATP exposure. Heterologously expressed P2X2, P2X2/P2X3 and P2X4 channels as well as native neuronal P2X channels possess various combinations of mono- or biphasic responses and permeability changes, measured by NMDG+ and fluorescent dye. Furthermore, in P2X4 receptors, this ability to alter ion selectivity can be increased or decreased by altering an amino-acid residue thought to line the ion permeation pathway, identifying a region that governs this activity-dependent change.

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Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1999 Nature America Inc. Received 30 November 1998, Accepted 20 January 1999. Thanks to I. Chessell (GlaxoWellcome, UK) for providing P2X cDNA clones. The authors are grateful to H. Li, S. McKinney and J. Sydes for assistance with preparation of oocytes and neurons and for advice on the YO-PRO-1 experiments, and to other members of the group for comments. This work was supported by the National Institutes of Health (NS-11756), a Wellcome Trust (UK) Prize Travelling Fellowship to B.S.K. and a Caltech Summer Undergraduate Research Fellowship to X.R.B.
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Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20150414-095506624
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56626
Deposited By: Tony Diaz
Deposited On:15 Apr 2015 04:43
Last Modified:10 Nov 2021 21:01

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