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Symmetrical Mutant Phenotypes of the Receptor EphB4 and Its Specific Transmembrane Ligand ephrin-B2 in Cardiovascular Development

Gerety, Sebastian S. and Wang, Hai U. and Chen, Zhou-Feng and Anderson, David J. (1999) Symmetrical Mutant Phenotypes of the Receptor EphB4 and Its Specific Transmembrane Ligand ephrin-B2 in Cardiovascular Development. Molecular Cell, 4 (3). pp. 403-414. ISSN 1097-2765. doi:10.1016/S1097-2765(00)80342-1. https://resolver.caltech.edu/CaltechAUTHORS:20150414-112327667

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Abstract

Ephrin-B2 is a transmembrane ligand that is specifically expressed on arteries but not veins and that is essential for cardiovascular development. However, ephrin-B2 is also expressed in nonvascular tissues and interacts with multiple EphB class receptors expressed in both endothelial and nonendothelial cell types. Thus, the identity of the relevant receptor for ephrin-B2 and the site(s) where these molecules interact to control angiogenesis were not clear. Here we show that EphB4, a specific receptor for ephrin-B2, is exclusively expressed by vascular endothelial cells in embryos and is preferentially expressed on veins. A targeted mutation in EphB4 essentially phenocopies the mutation in ephrin-B2. These data indicate that ephrin-B2–EphB4 interactions are intrinsically required in vascular endothelial cells and are consistent with the idea that they mediate bidirectional signaling essential for angiogenesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S1097-2765(00)80342-1DOIArticle
ORCID:
AuthorORCID
Anderson, David J.0000-0001-6175-3872
Additional Information:© 1999 by Cell Press. Under an Elsevier user license. Received August 6, 1999; revised August 31, 1999. We thank Emma Dormand for help with confocal microscopy and support, Scott Fraser for access to confocal microscopes, Shirley Pease and the staff of the Transgenic Animal Facility at Caltech for assistance with animal husbandry, Richard Behringer for AB-1 ES and STO 76/7 feeder cells, Gaby Mosconi for lab management, Lan Dinh and Hieu Thi Phan for technical support, and G. Mancuso for administrative assistance. D. J. A. is an Investigator of the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:3
DOI:10.1016/S1097-2765(00)80342-1
Record Number:CaltechAUTHORS:20150414-112327667
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150414-112327667
Official Citation:Sebastian S Gerety, Hai U Wang, Zhou-Feng Chen, David J Anderson, Symmetrical Mutant Phenotypes of the Receptor EphB4 and Its Specific Transmembrane Ligand ephrin-B2 in Cardiovascular Development, Molecular Cell, Volume 4, Issue 3, September 1999, Pages 403-414, ISSN 1097-2765, http://dx.doi.org/10.1016/S1097-2765(00)80342-1. (http://www.sciencedirect.com/science/article/pii/S1097276500803421)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56633
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2015 19:52
Last Modified:10 Nov 2021 21:02

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