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Identification by Differential RT-PCR of a Novel Paired Homeodomain Protein Specifically Expressed in Sensory Neurons and a Subset of Their CNS Targets

Saito, Tetsuichiro and Greenwood, Amy and Sun, Qi and Anderson, David J. (1995) Identification by Differential RT-PCR of a Novel Paired Homeodomain Protein Specifically Expressed in Sensory Neurons and a Subset of Their CNS Targets. Molecular and Cellular Neuroscience, 6 (3). pp. 280-292. ISSN 1044-7431. doi:10.1006/mcne.1995.1022. https://resolver.caltech.edu/CaltechAUTHORS:20150414-134642549

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Abstract

Sensory neurons are a major derivative of the neural crest for which there have been no definitive molecular markers in mammals. We have developed a method that combines differential hybridization with degenerate RT-PCR to rapidly screen gene families for members exhibiting differential expression among tissues or cell types. We used this approach to search for transcription factor-encoding genes specifically expressed in mammalian sensory neurons. A novel paired homeodomain protein, called DRG11, was identified. DRG11 is expressed in most sensory neurons, including trkA-expressing neurons, but not in glia or sympathetic neurons. Unexpectedly, it is also expressed in the dorsal horn of the spinal cord, a region to which NGF-dependent sensory neurons project. These data suggest that DRG11 is not only a useful marker for sensory neurons, but may also function in the establishment or maintenance of connectivity between some of these neurons and their central nervous system targets.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/mcne.1995.1022DOIArticle
ORCID:
AuthorORCID
Anderson, David J.0000-0001-6175-3872
Additional Information:© 1996 Academic Press. We thank Steven Padilla for technical assistance, Susan Ou, Ling Wang, and Liching Lo for advice and help in the production of monoclonal antibodies, Andy Groves for advice on immunostaining procedures, and Lukas Sommer and Kai Zinn for their helpful comments on the manuscript. D.J.A is an Associate Investigator of the Howard Medical Institute.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:3
DOI:10.1006/mcne.1995.1022
Record Number:CaltechAUTHORS:20150414-134642549
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150414-134642549
Official Citation:Tetsuichiro Saito, Amy Greenwood, Qi Sun, David J. Anderson, Identification by Differential RT-PCR of a Novel Paired Homeodomain Protein Specifically Expressed in Sensory Neurons and a Subset of Their CNS Targets, Molecular and Cellular Neuroscience, Volume 6, Issue 3, June 1995, Pages 280-292, ISSN 1044-7431, http://dx.doi.org/10.1006/mcne.1995.1022. (http://www.sciencedirect.com/science/article/pii/S1044743185710226)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56642
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Apr 2015 21:03
Last Modified:10 Nov 2021 21:02

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