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Antibody Markers Identify a Common Progenitor to Sympathetic Neurons and Chromaffin Cells in vivo and Reveal the Timing of Commitment to Neuronal Differentiation in the Sympathoadrenal Lineage

Anderson, David J. and Carnahan, Josette F. and Michelsohn, Arie and Patterson, Paul H. (1991) Antibody Markers Identify a Common Progenitor to Sympathetic Neurons and Chromaffin Cells in vivo and Reveal the Timing of Commitment to Neuronal Differentiation in the Sympathoadrenal Lineage. Journal of Neuroscience, 11 (11). pp. 3507-3519. ISSN 0270-6474. PMCID PMC6575531. https://resolver.caltech.edu/CaltechAUTHORS:20150414-143643042

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Abstract

Using specific antibody markers and double-label immunofluorescence microscopy, we have followed the fate of progenitor cells in the sympathoadrenal (SA) sublineage of the neural crest in developing rat embryos. Such progenitors are first recognizable in the primordial sympathetic ganglia at embryonic day 11.5 (E11.5), when they express tyrosine hydroxylase. At this stage, the progenitors also coexpress neuronal markers such as SCG 10 and neurofilament, together with a series of chromaffin cell markers called SA 1–5 (Carnhan and Patterson, 1991 a). The observation of such doubly labeled cells is consistent with the hypothesis that these cells represent a common progenitor to sympathetic neurons and adrenal chromaffin cells. Subsequent to E 11.5, expression of the chromaffin markers is extinguished in the sympathetic ganglia but retained by cells within the adrenal gland. Concomitant with the loss of the SA 1-5 immunoreactivity in sympathetic ganglia, a later sympathetic neuron-specific marker, B2, appears. In dissociated cell suspensions, some B2+ cells that coexpress SA 1 are seen. This implies a switch in the antigenic phenotype of developing sympathetic neurons, rather than a replacement of one cell population by another. The SA 1--B2 transition does not occur for the majority of cells within the adrenal primordium. In vitro, most B2+ cells fail to differentiate into chromaffin cells in response to glucocorticoid. Instead, they continue to extend neurites and then die. Taken together, these data imply that the SA 1--B2 transition correlates with a loss of competence to respond to an inducer of chromaffin differentiation. Thus, the development of SA derivatives is controlled both by environmental signals and by changes in the ability of differentiating cells to respond to such signals.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/JNEUROSCI.11-11-03507.1991DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6575531PubMed CentralArticle
ORCID:
AuthorORCID
Anderson, David J.0000-0001-6175-3872
Additional Information:© 1991 Society for Neuroscience. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Jan. 25, 1991; revised May 1, 1991; accepted June 11, 1991. We are grateful to Joan Roach for assistance with histology and immunofluorescence, to Shelly Diamond for help with cell sorting, and to Li-Ching Lo for the preparation of anti-SCG 10 antibodies. We also thank Dr. Jane Dodd (Columbia University, New York) for the generous gift of monoclonal antibody B2 and for helpful suggestions. We thank members of the Anderson laboratory for helpful discussions. This work was supported by NIH Grant NS23476 to DJ.A., and an NINDS grant (Javits Neuroscience Investigator Award) and a McKnight Foundation Neuroscience Research Project Award to P.H.P. D.J.A. is an Assistant Investigator of the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
NIHNS23476
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
McKnight FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC6575531
Record Number:CaltechAUTHORS:20150414-143643042
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150414-143643042
Official Citation:Antibody markers identify a common progenitor to sympathetic neurons and chromaffin cells in vivo and reveal the timing of commitment to neuronal differentiation in the sympathoadrenal lineage. DJ Anderson, JF Carnahan, A Michelsohn, PH Patterson. Journal of Neuroscience 1 November 1991, 11 (11) 3507-3519; DOI: 10.1523/JNEUROSCI.11-11-03507.1991
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56649
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:15 Apr 2015 04:23
Last Modified:30 Oct 2019 17:21

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