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The induction of a neural-specific gene, SCG10, by nerve growth factor in PC12 cells is transcriptional, protein synthesis dependent, and glucocorticoid inhibitable

Stein, Reuven and Orit, Sigmond and Anderson, David J. (1988) The induction of a neural-specific gene, SCG10, by nerve growth factor in PC12 cells is transcriptional, protein synthesis dependent, and glucocorticoid inhibitable. Developmental Biology, 127 (2). pp. 316-325. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20150414-153531383

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Abstract

We describe features of the regulation of a neural-specific gene, SCG10, which is induced by nerve growth factor (NGF) during the neuronal differentiation of the rat pheochromocytoma cell line PC12. Induction of SCG10 mRNA occurs within 12–24 hr of exposure to NGF, is sustained in the continued presence of the neurotrophic factor, and involves a mechanism that is, at least in part, transcriptional. Unlike the rapid, transient transcriptional activations of genes such as c-fos, SCG10 induction requires ongoing protein synthesis, suggesting the participation of a de novo synthesized regulatory protein in mediating the effects of NGF on this gene. Although c-fos itself may play this role, its induction is clearly insufficient to cause an induction of SCG10. NGF, FGF, and, to a lesser extent, phorbol esters induced SCG10, whereas EGF and dibutyryl cAMP did not. In these characteristics, SCG10 induction appears to constitute a reliable molecular index of the transcription-dependent neuronal differentiation induced by NGF. Glucocorticoids, which inhibit NGF-induced neurite outgrowth from normal primary chromaffin cells, partially blocked SCG10 induction in PC12 cells. A reciprocal pattern of regulation by NGF and glucocorticoids was observed for tyrosine hydroxylase mRNA. These data suggest that environmental signals such as NGF may act on specific genes, both positively and negatively, to control the choice of alternative fates by developing neural crest cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(88)90318-1 DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160688903181PublisherArticle
Additional Information:© 1988 by Academic Press, Inc. Accepted February 16, 1988. This work was supported by NIH Grant No. NS23476-01 and an NSF Presidential Young Investigator Award to D.J.A., and by grants from the Moise and Frida Eskanasy Cancer Research Center and the Center for Absorption in Science to R.S.
Funders:
Funding AgencyGrant Number
NIHNS23476-01
NSF Presidential Young Investigator AwardUNSPECIFIED
Moise and Frida Eskanasy Cancer Research CenterUNSPECIFIED
Center for Absorption in ScienceUNSPECIFIED
Record Number:CaltechAUTHORS:20150414-153531383
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150414-153531383
Official Citation:Reuven Stein, Sigmond Orit, David J. Anderson, The induction of a neural-specific gene, SCG10, by nerve growth factor in PC12 cells is transcriptional, protein synthesis dependent, and glucocorticoid inhibitable, Developmental Biology, Volume 127, Issue 2, June 1988, Pages 316-325, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(88)90318-1. (http://www.sciencedirect.com/science/article/pii/0012160688903181)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56653
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Apr 2015 04:16
Last Modified:15 Apr 2015 04:16

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