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Molecular dissection of the amygdala and its relevance to autism

Zirlinger, M. and Anderson, D. (2003) Molecular dissection of the amygdala and its relevance to autism. Genes, Brain and Behavior, 2 (5). pp. 282-294. ISSN 1601-1848. doi:10.1034/j.1601-183X.2003.00039.x.

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The limbic system, and in particular the amygdala, have been implicated in autism. The amygdala is a complex structure that in rodents consists of at least 12 different nuclei or subnuclei. A comparative analysis of amygdala neuroanatomy in normal vs. autistic brains would be aided by the availability of molecular markers to unambiguously recognize these different amygdala substructures. Here we report on the development of methods to identify genes enriched in the central, lateral and medial nuclei of the rodent amygdala. Our results suggest that laser-capture microdissection of specific amygdala subnuclei, when combined with linear amplification of cRNA probes for oligonucleotide microarray hybridization, can efficiently identify genes whose expression is confined to these substructures. Importantly, many of these genes were missed in previous gene expression-profiling experiments using whole amygdala tissue. The isolation of human orthologs of these subnucleus-specific genes, and/or the application of these methods directly to human tissue, may provide useful markers for characterizing neuropathological correlates of autism, as well as for identifying molecular differences between normal and autistic brains.

Item Type:Article
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Anderson, D.0000-0001-6175-3872
Additional Information:© 2003 Blackwell Munksgaard. Received 23 May 2003, revised 27 June 2003, accepted for publication 8 July 2003. We thank G. Kreiman for help with software development and G. Mosconi for managerial assistance. Supported by the Mettler fund on autism, a gift from Merck, and NIMH grant MH62825-01.
Funding AgencyGrant Number
Mettler Fund on AutismUNSPECIFIED
National Institute of Mental Health (NIMH)MH62825-01
Subject Keywords:affymetrix; animal model; chips; in situ hybridization; LCM; validation
Issue or Number:5
Record Number:CaltechAUTHORS:20150415-090909564
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Official Citation:Zirlinger, M. and Anderson, D. (2003), Molecular dissection of the amygdala and its relevance to autism. Genes, Brain and Behavior, 2: 282–294. doi: 10.1034/j.1601-183X.2003.00039.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56668
Deposited By: Tony Diaz
Deposited On:16 Apr 2015 04:23
Last Modified:10 Nov 2021 21:02

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