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The use of Epstein-Barr virus-based shuttle vectors in rat PC12 cells

Lindenboim, Liora and Anderson, David and Stein, Reuven (1997) The use of Epstein-Barr virus-based shuttle vectors in rat PC12 cells. Cellular and Molecular Neurobiology, 17 (1). pp. 119-127. ISSN 0272-4340. https://resolver.caltech.edu/CaltechAUTHORS:20150415-092309883

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Abstract

1. The rat pheochromocytoma PC12 cell line has been a commonly used model for studies of neuronal development, function, and death. Thus the abilityto transfect PC12 cells in an efficient manner and to manipulate their gene expression would enhance the usefulness of these cells. 2. We demonstrate that EBV-based vectors provide a useful expression system for gene manipulation in rat PC12 cells. 3. The EBV-based vectors replicate episomally in PC12 cells for at least 2 months, as evidence by their recovery from the transfected cells and by the digestion of the episomal plasmid with the isoschizomer MboI and DpnI restriction enzymes. 3. PC12 cells are efficiently transfected by EBV-based vectors both transiently and stably. 4. Transfection of PC12 cells with an EBV-based vector containing tau cDNA in the antisense orientation resulted in a decrease in the level of tau protein in the transfected cells. 5. The results demonstrate that EBV-based vectors can be a useful expression system for gene manipulation in PC12 cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1023/A:1026385206078 DOIArticle
http://link.springer.com/article/10.1023%2FA%3A1026385206078PublisherArticle
ORCID:
AuthorORCID
Anderson, David0000-0001-6175-3872
Additional Information:© 1997 Plenum Publishing Corporation. Received January 12, 1995; accepted January 2, 1996. This work was supported by Grant 90-00347 from the United States-Israel Binational Science Foundation (Jerusalem, Israel) and by the Herczeg Institute of Aging Foundation.
Funders:
Funding AgencyGrant Number
Binational Science Foundation (USA-Israel)90-00347
Herczeg Institute of Aging FoundationUNSPECIFIED
Subject Keywords:PC12 cells; Epstein-Barr virus-based vector; transfection
Issue or Number:1
Record Number:CaltechAUTHORS:20150415-092309883
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150415-092309883
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56670
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Apr 2015 04:22
Last Modified:09 Mar 2020 13:18

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