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Biosynthesis of Acetylcholine Receptor in Vitro

Anderson, David J. and Blobel, Günter (1983) Biosynthesis of Acetylcholine Receptor in Vitro. In: Biomembranes Part J: Membrane Biogenesis: Assembly and Targeting. Methods in Enzymology. No.96. Academic Press , New York, NY, pp. 367-378. ISBN 978-0-12-181996-5. https://resolver.caltech.edu/CaltechAUTHORS:20150415-094809722

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Abstract

Torpedo acetylcholine receptor is accessible to analysis by cell-free protein synthesis, like other membrane proteins studied previously. The factors deserving particular attention in this system are the small amounts of messenger RNA contained in electroplax tissue, the inherent complexity of an oligomeric membrane protein (necessitating the use of subunit-specific antibodies), the anomalous electrophoretic behavior of the primary translation products (attributable in part to the high—SH content of sAChRs), and the multiple glycosylated forms that appear upon processing by microsomal membranes. Although not all these complexities can be satisfactorily explained, they have been empirically characterized and can be well controlled if the appropriate procedures are followed.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0076-6879(83)96033-0DOIArticle
http://www.sciencedirect.com/science/article/pii/S0076687983960330PublisherArticle
ORCID:
AuthorORCID
Anderson, David J.0000-0001-6175-3872
Additional Information:© 1983 Academic Press, Inc.
Series Name:Methods in Enzymology
Issue or Number:96
Record Number:CaltechAUTHORS:20150415-094809722
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150415-094809722
Official Citation:David J. Anderson, Günter Blobel, [29] Biosynthesis of acetylcholine receptor in Vitro, In: Sidney Fleischer, Becca Fleischer, Editor(s), Methods in Enzymology, Academic Press, 1983, Volume 96, Pages 367-378, ISSN 0076-6879, ISBN 9780121819965, http://dx.doi.org/10.1016/S0076-6879(83)96033-0. (http://www.sciencedirect.com/science/article/pii/S0076687983960330)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56673
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 Apr 2015 04:21
Last Modified:09 Mar 2020 13:18

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