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Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells

Lo, Li-Ching and Johnson, Jane E. and Wuenschell, Carol W. and Saito, Tetsuichiro and Anderson, David J. (1991) Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells. Genes and Development, 5 (9). pp. 1524-1537. ISSN 0890-9369. https://resolver.caltech.edu/CaltechAUTHORS:20150415-111341773

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Abstract

Using monoclonal antibodies, we have examined the expression pattern of MASH1, a basic helix-loop-helix protein that is a mammalian homolog of the Drosophila achaete-scute proteins. In Drosophila, achaete-scute genes are required for the determination of a subset of neurons. In the rat embryo, MASH1 expression is confined to subpopulations of neural precursor cells. The induction of MASH1 precedes, but is extinguished upon, overt neuronal differentiation. MASH1 is expressed in the forebrain by spatially restricted domains of neuroepithelium and in the peripheral nervous system exclusively by precursors of sympathetic and enteric neurons. The features of early and transient expression, in spatially restricted subpopulations of neural precursors, are similar to those observed for achaete-scute. Thus, the amino acid sequence conservation between MASH1 and achaete-scute is reflected in a parallel conservation of cell type specificity of expression, similar to the case of mammalian MyoD and Drosophila nautilus. These data support the idea that helix-loop-helix proteins may represent an evolutionarily conserved family of cell-type determination genes, of which MASH1 is the first neural-specific member identified in vertebrates.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/gad.5.9.1524DOIArticle
http://genesdev.cshlp.org/content/5/9/1524PublisherArticle
Additional Information:© 1991 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/) Received June 21, 1991; accepted July 10, 1991. We express our gratitude for the central contribution and expert performance of Susan Ou of the Caltech Monoclonal Antibody Facility, under the direction of Paul Patterson, in producing monoclonal antibodies to MASHl. We thank Karina Cramer for help with the serial reconstruction, the Caltech Graphic Arts facility for expert photographic assistance, Helen Walsh for typing, and Barbara Wold, Paul Patterson, John Montgomery, Antonio Garcia-Bellido, and Thomas Jessell for their comments on the manuscript. This work was supported by the Howard Hughes Medical Institute, a Sloan Foundation Faculty Fellowship in Neurosciences (to D.J.A.), a National Science Foundation Presidential Young Investigator Award, and a Searle Scholars Award. J.E.J. is a postdoctoral fellow of the Muscular Dystrophy Association. The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
NSFUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Muscular Dystrophy AssociationUNSPECIFIED
Subject Keywords:Helix-loop-helix proteins; neuronal determination; forebrain; achaete-scute genes; nuclear regulatory factor; neural crest development
Issue or Number:9
Record Number:CaltechAUTHORS:20150415-111341773
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150415-111341773
Official Citation:Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells. L C Lo, J E Johnson, C W Wuenschell, T Saito, and D J Anderson Genes Dev. September 1991 5: 1524-1537; doi:10.1101/gad.5.9.1524
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56682
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 Apr 2015 04:17
Last Modified:03 Oct 2019 08:16

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