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Lack of in Vivo Antibody Dependent Cellular Cytotoxicity with Antibody Containing Gold Nanoparticles

Ahmed, Marya and Pan, Dorothy W. and Davis, Mark E. (2015) Lack of in Vivo Antibody Dependent Cellular Cytotoxicity with Antibody Containing Gold Nanoparticles. Bioconjugate Chemistry, 26 (5). pp. 812-816. ISSN 1043-1802. PMCID PMC4445771. doi:10.1021/acs.bioconjchem.5b00139.

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Antibody-dependent cellular cytotoxicity (ADCC) is a cytolytic mechanism that can elicit in vivo antitumor effects and can play a significant role in the efficacy of antibody treatments for cancer. Here, we prepared cetuximab, panitumumab, and rituximab containing gold nanoparticles and investigated their ability to produce an ADCC effect in vivo. Cetuximab treatment of EGFR-expressing H1975 tumor xenografts showed significant tumor regression due to the ADCC activity of the antibody in vivo, while the control antibody, panitumumab, did not. However, all three antibody containing nanoparticles are not able to suppress tumor growth in the same in vivo mouse model. The antibody containing nanoparticles localized in the tumors and did not suppress the immune function of the animals, so the lack of tumor growth suppression of the cetuximab containing nanoparticle suggests that immobilizing antibodies onto a nanoparticle significantly decreases the ability of the antibody to promote an ADCC response.

Item Type:Article
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URLURL TypeDescription Information CentralArticle
Davis, Mark E.0000-0001-8294-1477
Additional Information:© 2015 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Received: March 17, 2015; Revised: April 9, 2015; Publication Date (Web): April 16, 2015. We thank Kristin Anderson for performing MTS cell viability assays, Mona Shahgholi for MALDI-TOF-MS training, and Andres Collazo (Beckman Imaging Center) for confocal microscopy trainning. This work was supported by the National Cancer Institute Grant CA 151819 and the National Science and Engineering Research Council of Canada (NSERC) (fellowship for M.A.).
Funding AgencyGrant Number
National Cancer InstituteCA 151819
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC4445771
Record Number:CaltechAUTHORS:20150420-092421971
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Official Citation:Lack of in Vivo Antibody Dependent Cellular Cytotoxicity with Antibody Containing Gold Nanoparticles Marya Ahmed, Dorothy W. Pan, and Mark E. Davis Bioconjugate Chemistry 2015 26 (5), 812-816 DOI: 10.1021/acs.bioconjchem.5b00139
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56759
Deposited By: Tony Diaz
Deposited On:20 Apr 2015 19:12
Last Modified:10 Nov 2021 21:03

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