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Plant stem cell maintenance by transcriptional cross-regulation of related receptor kinases

Nimchuk, Zachary L. and Zhou, Yun and Tarr, Paul T. and Peterson, Brenda A. and Meyerowitz, Elliot M. (2015) Plant stem cell maintenance by transcriptional cross-regulation of related receptor kinases. Development, 142 (6). pp. 1043-1049. ISSN 0950-1991. PMCID PMC4360179. doi:10.1242/dev.119677. https://resolver.caltech.edu/CaltechAUTHORS:20150420-095210564

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Abstract

The CLAVATA3 (CLV3)-CLAVATA1 (CLV1) ligand-receptor kinase pair negatively regulates shoot stem cell proliferation in plants. clv1 null mutants are weaker in phenotype than clv3 mutants, but the clv1 null phenotype is enhanced by mutations in the related receptor kinases BARELY ANY MERISTEM 1, 2 and 3 (BAM1, 2 and 3). The basis of this genetic redundancy is unknown. Here, we demonstrate that the apparent redundancy in the CLV1 clade is in fact due to the transcriptional repression of BAM genes by CLV1 signaling. CLV1 signaling in the rib meristem (RM) of the shoot apical meristem is necessary and sufficient for stem cell regulation. CLV3-CLV1 signaling in the RM represses BAM expression in wild-type Arabidopsis plants. In clv1 mutants, ectopic BAM expression in the RM partially complements the loss of CLV1. BAM regulation by CLV1 is distinct from CLV1 regulation of WUSCHEL, a proposed CLV1 target gene. In addition, quadruple receptor mutants are stronger in phenotype than clv3, pointing to the existence of additional CLV1/BAM ligands. These data provide an explanation for the genetic redundancy seen in the CLV1 clade and reveal a novel feedback operating in the control of plant stem cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.119677DOIArticle
http://dev.biologists.org/content/142/6/1043PublisherArticle
http://dev.biologists.org/content/142/6/1043/suppl/DC1PublisherSupplementary Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4360179/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2015 The Company of Biologists, Ltd. Received 9 November 2014; Accepted 27 January 2015. We thank Arnavaz Garda for lab support; Dr Steven Clark for providing the La-er/Col-0 bam1-1 bam2-1 bam3-2 seed; and Dr Rü diger Simon for providing the clv3-9 allele. This work was funded by a National Institutes of Health (NIH) grant [1R01GM086639] to E.M.M.; by funds from the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation [through grant GBMF3406]; and supported in part by start-up funds provided by Virginia Tech to Z.L.N. Deposited in PMC for release after 6 months.
Funders:
Funding AgencyGrant Number
NIH1R01GM086639
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationGBMF3406
Virginia TechUNSPECIFIED
Subject Keywords:CLV1; Plant development; Receptor kinase; Stem cells; Arabidopsis thaliana
Issue or Number:6
PubMed Central ID:PMC4360179
DOI:10.1242/dev.119677
Record Number:CaltechAUTHORS:20150420-095210564
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150420-095210564
Official Citation:Nimchuk, Z. L., Zhou, Y., Tarr, P. T., Peterson, B. A., & Meyerowitz, E. M. (2015). Plant stem cell maintenance by transcriptional cross-regulation of related receptor kinases. Development, 142(6), 1043-1049. doi: 10.1242/dev.119677
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56765
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:21 Apr 2015 22:07
Last Modified:10 Nov 2021 21:03

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