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FGF receptor signalling is required to maintain neural progenitors during Hensen's node progression

Mathis, Luc and Kulesa, Paul M. and Fraser, Scott E. (2001) FGF receptor signalling is required to maintain neural progenitors during Hensen's node progression. Nature Cell Biology, 3 (6). pp. 559-566. ISSN 1465-7392. doi:10.1038/35078535. https://resolver.caltech.edu/CaltechAUTHORS:20150422-085227054

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Abstract

Previous analyses of labelled clones of cells within the developing nervous system of the mouse have indicated that descendants are initially dispersed rostrocaudally followed by more local proliferation, which is consistent with the progressing node's contributing descendants from a resident population of progenitor cells as it advances caudally. Here we electroporated an expression vector encoding green fluorescent protein into the chicken embryo near Hensen's node to test and confirm the pattern inferred in the mouse. This provides a model in which a proliferative stem zone is maintained in the node by a localized signal; those cells that are displaced out of the stem zone go on to contribute to the growing axis. To test whether fibroblast growth factor (FGF) signalling could be involved in the maintenance of the stem zone, we co-electroporated a dominant-negative FGF receptor with a lineage marker, and found that it markedly alters the elongation of the spinal cord primordium. The results indicate that FGF receptor signalling promotes the continuous development of the posterior nervous system by maintaining presumptive neural progenitors in the region near Hensen's node. This offers a potential explanation for the mixed findings on FGF in the growth and patterning of the embryonic axis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/35078535 DOIArticle
http://www.nature.com/ncb/journal/v3/n6/full/ncb0601_559.htmlPublisherArticle
http://www.nature.com/ncb/journal/v3/n6/suppinfo/ncb0601_559_S1.htmlPublisherSupplementary Information
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2001 Macmillan Magazines Ltd. Received 11 July 2000; Revised 23 January 2001; Accepted 5 March 2001; Published 17 May 2001. We thank R. Lansford, E. Dorman, E. Amaya and L. Niswander for reagents, M. Bronner-Fraser, M. Garcia-Castro, A. Knecht and H. McBride for comments on the manuscript, members of the Fraser laboratory for advice, and J. Horn for his help with electroporation. L.M. is the recipient of an EMBO long-term fellowship; P.M.K. is a participant in the California Institute of Technology Initiative in Computational Molecular Biology, which is funded by a Burroughs Wellcome Fund Interfaces award.
Funders:
Funding AgencyGrant Number
EMBO FellowshipUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
Issue or Number:6
DOI:10.1038/35078535
Record Number:CaltechAUTHORS:20150422-085227054
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150422-085227054
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56854
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Apr 2015 16:38
Last Modified:10 Nov 2021 21:04

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