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Electron transfer and DNA replication: Assessing the functional role of the yeast DNA polymerase δ [4Fe- 4S] 2+ cluster

Bartels, Phillip L. and Stodola, Joseph L. and Burgers, Peter M. J. and Barton, Jacqueline K. (2015) Electron transfer and DNA replication: Assessing the functional role of the yeast DNA polymerase δ [4Fe- 4S] 2+ cluster. Abstracts of Papers of the American Chemical Society, 249 . BIOL-129. ISSN 0065-7727. https://resolver.caltech.edu/CaltechAUTHORS:20150423-132051717

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Abstract

Eukaryotic B- family DNA polymerases have recently been shown to contain a conserved [4Fe- 4S]2+ cluster in the C- terminus of the catalytic subunit. This cofactor has been most completely characterized in yeast DNA polymerase δ (Pol δ) , the enzyme responsible for lagging strand DNA synthesis. Multiple lines of evidence point to a role for the cluster beyond structural integrity, but the nature of this function is not obvious. Clues to what this function might be come from previous work in the Barton lab, which showed that [4Fe- 4S]2+ clusters in bacterial base excision repair enzymes undergo a shift in potential favoring oxidn. upon binding to DNA, allowing them to utilize DNA- mediated redox signaling to coordinate their activities and find their targets. Building on this earlier work, we have investigated the capability of the Pol δ [4Fe- 4S]2+ cluster to undergo reversible electron transfer using DNA- modified electrodes, and have designed assays to test the effect of redox state on enzymic activity.


Item Type:Article
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http://www.acs.org/content/acs/en/meetings/spring-2015.htmlOrganizationConference Website
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2015 American Chemical Society.
Record Number:CaltechAUTHORS:20150423-132051717
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150423-132051717
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56923
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Apr 2015 21:24
Last Modified:03 Oct 2019 08:18

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