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DNA-mediated redox signaling by UvrC

Grodick, Michael A. and Silva, Rebekah M. and Zhou, Andy and Barton, Jacqueline K. (2015) DNA-mediated redox signaling by UvrC. Abstracts of Papers of the American Chemical Society, 249 . BIOL-113. ISSN 0065-7727.

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The E. coli UvrC protein is an integral part of the DNA nucleotide excision repair pathway. Our lab. has focused on studies of redox DNA signaling among DNA repair proteins contg. 4Fe- 4S clusters. We have now explored whether UvrC may also contain a 4Fe- 4S cluster. A new purifn. route was developed to express an MBP- UvrC fusion. The purified protein shows a UVvisible absorbance spectrum consistent with that of a 4Fe- 4S cluster. ESR expts. of reduced UvrC give rise to a signal indicative of a 4Fe- 4S cluster. Electrochem. expts. indicate that the protein is electrochem. active on DNA- modified gold electrodes. Finally, genetic methods to study possible DNA- mediated signaling between UvrC and other DNA- processing enzymes contg. 4Fe- 4S clusters are being examd.

Item Type:Article
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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2015 American Chemical Society.
Record Number:CaltechAUTHORS:20150424-080918487
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56935
Deposited By: Tony Diaz
Deposited On:24 Apr 2015 16:15
Last Modified:03 Oct 2019 08:18

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