McGuirl, Michele A. and Lee, Jennifer C. and Lyubovitsky, Julia G. and Thanyakoop, Chalita and Richards, John H. and Gray, Harry B. and Winkler, Jay R. (2003) Cloning, heterologous expression, and characterization of recombinant class II cytochromes c from Rhodopseudomonas palustris. Biochimica et Biophysica Acta - General Subjects, 1619 (1). pp. 23-8. ISSN 0304-4165. doi:10.1016/S0304-4165(02)00437-3. https://resolver.caltech.edu/CaltechAUTHORS:20150424-155358251
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Abstract
The cytochrome (cyt) c′, cyt c_(556), and cyt c_2 genes from Rhodopseudomonas palustris have been cloned; recombinant cyt c′ and cyt c_(556) have been expressed, purified, and characterized. Unlike mitochondrial cyt c, these two proteins are structurally similar to cyt b_(562), in which the heme is embedded in a four-helix bundle. The hemes in both recombinant proteins form covalent thioether links to two Cys residues. UV/vis spectra of the Fe^(II) and Fe^(III) states of the recombinant cyts are identical with those of the corresponding native proteins. Equilibrium unfolding measurements in guanidine hydrochloride solutions confirm that native Fe^(II)-cyt c_(556) is more stable than the corresponding state of Fe^(III)-cyt c_(556) (ΔΔG_f°=22 kJ/mol).
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| Additional Information: | © 2002 Elsevier. Received 16 July 2002; received in revised form 9 September 2002; accepted 19 September 2002. The authors thank Linda Thöny-Meyer for the gift of plasmid pEC86, and A. Grant Mauk for the gift of plasmid pBPCYC1. This work was supported by NSF (MCB 9974477), NIH (GM16424, J.H.R.; GM020316-03, M.A.M.; GM07616, J.G.L), the Ralph M. Parsons Foundation (J.C.L.), and the Arnold and Mabel Beckman Foundation. | ||||||||||||||
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| Subject Keywords: | Cytochrome c′; Cytochrome c_(556); Protein folding; Rhodopseudomonas palustris; Spectroscopy | ||||||||||||||
| Issue or Number: | 1 | ||||||||||||||
| DOI: | 10.1016/S0304-4165(02)00437-3 | ||||||||||||||
| Record Number: | CaltechAUTHORS:20150424-155358251 | ||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150424-155358251 | ||||||||||||||
| Official Citation: | Michele A. McGuirl, Jennifer C. Lee, Julia G. Lyubovitsky, Chalita Thanyakoop, John H. Richards, Harry B. Gray, Jay R. Winkler, Cloning, heterologous expression, and characterization of recombinant class II cytochromes c from Rhodopseudomonas palustris, Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1619, Issue 1, 2 January 2003, Pages 23-28, ISSN 0304-4165, http://dx.doi.org/10.1016/S0304-4165(02)00437-3. (http://www.sciencedirect.com/science/article/pii/S0304416502004373) | ||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||
| ID Code: | 56981 | ||||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||||
| Deposited By: | George Porter | ||||||||||||||
| Deposited On: | 24 Apr 2015 23:07 | ||||||||||||||
| Last Modified: | 10 Nov 2021 21:06 |
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