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Electronic Spectroscopy of Gold(I) Pseudomonasaeruginosa Azurin Derivatives

McCleskey, T. Mark and Mizoguchi, Tadashi J. and Richards, John H. and Gray, Harry B. (1996) Electronic Spectroscopy of Gold(I) Pseudomonasaeruginosa Azurin Derivatives. Inorganic Chemistry, 35 (11). pp. 3434-3435. ISSN 0020-1669. http://resolver.caltech.edu/CaltechAUTHORS:20150424-155400474

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Abstract

The trigonal (His_2Cys) coordination of blue copper sites in proteins favors Cu(I) over Cu(II), as reflected in the relatively high Cu^(II/I) reduction potentials; and the rigid polypeptide environment minimizes Cu^(II/I) nuclear reorganization, thereby facilitating long-range electron-transfer reactions with donor and acceptor molecules. Although blue Cu(II) sites exhibit rich spectroscopic and magnetic properties, the corresponding Cu(I) proteins do not; indeed, the methods that can be employed to investigate d^(10) metal sites are very limited. Because recent work has shown that Au(I) has geometry-sensitive d-p absorptions and emissions, we are using this 5d^(10) ion to probe ligand interactions in the Cu(I) sites of proteins. Here we report the electronic spectroscopy of Au(I)-substituted wild-type (WT) Pseudomonas aeruginosa azurin as well as the Au(I) derivative of a mutant in which the methionine at position 121 has been replaced with glycine (Met121Gly).


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http://dx.doi.org/10.1021/ic951055iDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ic951055iPublisherArticle
Additional Information:© 1996 American Chemical Society. Received August 9, 1995. We thank Angelo Di Bilio, Bo Malmström, Vinny Miskowski, and Jay Winkler for helpful discussions. This work was supported by the National Institutes of Health (DK19038).
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NIHDK19038
Record Number:CaltechAUTHORS:20150424-155400474
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150424-155400474
Official Citation:Electronic Spectroscopy of Gold(I) Pseudomonas aeruginosa Azurin Derivatives T. Mark McCleskey,Tadashi J. Mizoguchi,John H. Richards,* and, and Harry B. Gray* Inorganic Chemistry 1996 35 (11), 3434-3435 DOI: 10.1021/ic951055i
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:56984
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:27 Apr 2015 15:20
Last Modified:27 Apr 2015 15:20

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