Hunkapiller, Michael W. and Forgac, Michael D. and Yu, Ellen Ho and Richards, John H. (1979) ^(13)C NMR studies of the binding of soybean trypsin inhibitor to trypsin. Biochemical and Biophysical Research Communications, 87 (1). pp. 25-31. ISSN 0006-291X. doi:10.1016/0006-291X(79)91642-5. https://resolver.caltech.edu/CaltechAUTHORS:20150428-071027468
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Abstract
NMR studies of the complex between trypsin and soybean trypsin inhibitor with 1-^(13)C-arginine and modified inhibitor with 1-^(13)C-lysine show that these complexes involve almost exclusively non-covalent binding of the inhibitor to the enzyme for trypsin/^(13)C-Lys-inhibitor at pH 6.5 and 8.1 and for trypsin/^(13)C-Arg-inhibitor at pH 5.0. At pH 7.1 for trypsin/^(13)C-Arg-inhibitor both non-covalent and acyl enzyme forms are observed. Under no conditions did we observe evidence for a tetrahedral adduct between enzyme and inhibitor.
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| Additional Information: | © 1979 by Academic Press. Inc. Received 17 January 1979. This work was supported by NIH grants GM 10218 and GM 16424. | |||||||||
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| Issue or Number: | 1 | |||||||||
| DOI: | 10.1016/0006-291X(79)91642-5 | |||||||||
| Record Number: | CaltechAUTHORS:20150428-071027468 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150428-071027468 | |||||||||
| Official Citation: | Michael W. Hunkapiller, Michael D. Forgac, Ellen Ho Yu, John H. Richards, 13C NMR studies of the binding of soybean trypsin inhibitor to trypsin, Biochemical and Biophysical Research Communications, Volume 87, Issue 1, 15 March 1979, Pages 25-31, ISSN 0006-291X, http://dx.doi.org/10.1016/0006-291X(79)91642-5. (http://www.sciencedirect.com/science/article/pii/0006291X79916425) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 57030 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | INVALID USER | |||||||||
| Deposited On: | 28 Apr 2015 19:15 | |||||||||
| Last Modified: | 10 Nov 2021 21:07 |
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