Goetze, A. M. and Richards, J. H. (1977) Magnetic resonance studies of the binding site interactions between phosphorylcholine and specific mouse myeloma immunoglobulin. Biochemistry, 16 (2). pp. 228-232. ISSN 0006-2960. doi:10.1021/bi00621a011. https://resolver.caltech.edu/CaltechAUTHORS:20150428-104629036
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Abstract
The interaction of phosphorylcholine-binding mouse myeloma protein M603 and the isotopically substituted hapten phosphoryl[rnethyl-^(13)C]choline has been investigated using ^(13)C and ^(31)P nuclear magnetic resonance (NMR) spectroscopy. Upon binding to antibody, upfield shifts of 0.7 and 1.5 ppm are observed for the hapten ^(13)C and ^(31)P resonances, respectively, and both spectra are in the "slow" exchange limit. Linewidth analysis indicates some immobilization of the phosphate group but essentially unrestricted methyl group rotation for the bound hapten. Hapten-antibody dissociation rate constants of 10 and 38 s^(-1) are calculated from ^(13)C and ^(31)P NMR spectra, respectively, suggesting the possibility of differential dissociation rates for the two opposing ends of the phosphorylcholine molecule. The NMR data are entirely consistent with the known x-ray structure of the M603 Fab'-phosphorylcholine complex (Segal, D. M., Padlan, E. A., Cohen, G. H., Rudikoff, S., Potter, M., and Davies, D.R. (1974), Proc. Natl. Acad. Sci. U.S.A. 71, 4298).
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| Additional Information: | © 1977 American Chemical Society. Received July 22. 1976. Supported by grants from the President's Fund of Caltech and the United States Public Health Service (NIGM-16424 and NIAI 01339). | |||||||||
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| Issue or Number: | 2 | |||||||||
| DOI: | 10.1021/bi00621a011 | |||||||||
| Record Number: | CaltechAUTHORS:20150428-104629036 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150428-104629036 | |||||||||
| Official Citation: | Magnetic resonance studies of the binding site interactions between phosphorylcholine and specific mouse myeloma immunoglobulin A. M. Goetze and J. H. Richards Biochemistry 1977 16 (2), 228-232 DOI: 10.1021/bi00621a011 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 57044 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 28 Apr 2015 18:28 | |||||||||
| Last Modified: | 10 Nov 2021 21:07 |
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