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Magnetic resonance studies of the binding site interactions between phosphorylcholine and specific mouse myeloma immunoglobulin

Goetze, A. M. and Richards, J. H. (1977) Magnetic resonance studies of the binding site interactions between phosphorylcholine and specific mouse myeloma immunoglobulin. Biochemistry, 16 (2). pp. 228-232. ISSN 0006-2960. http://resolver.caltech.edu/CaltechAUTHORS:20150428-104629036

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Abstract

The interaction of phosphorylcholine-binding mouse myeloma protein M603 and the isotopically substituted hapten phosphoryl[rnethyl-^(13)C]choline has been investigated using ^(13)C and ^(31)P nuclear magnetic resonance (NMR) spectroscopy. Upon binding to antibody, upfield shifts of 0.7 and 1.5 ppm are observed for the hapten ^(13)C and ^(31)P resonances, respectively, and both spectra are in the "slow" exchange limit. Linewidth analysis indicates some immobilization of the phosphate group but essentially unrestricted methyl group rotation for the bound hapten. Hapten-antibody dissociation rate constants of 10 and 38 s^(-1) are calculated from ^(13)C and ^(31)P NMR spectra, respectively, suggesting the possibility of differential dissociation rates for the two opposing ends of the phosphorylcholine molecule. The NMR data are entirely consistent with the known x-ray structure of the M603 Fab'-phosphorylcholine complex (Segal, D. M., Padlan, E. A., Cohen, G. H., Rudikoff, S., Potter, M., and Davies, D.R. (1974), Proc. Natl. Acad. Sci. U.S.A. 71, 4298).


Item Type:Article
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URLURL TypeDescription
http://dx.doi.org/10.1021/bi00621a011DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi00621a011PublisherArticle
Additional Information:© 1977 American Chemical Society. Received July 22. 1976. Supported by grants from the President's Fund of Caltech and the United States Public Health Service (NIGM-16424 and NIAI 01339).
Funders:
Funding AgencyGrant Number
Caltech President’s FundUNSPECIFIED
United States Public Health ServiceNIGM-16424
United States Public Health ServiceNIAI 01339
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Caltech Church Laboratory of Chemical Biology5380
Record Number:CaltechAUTHORS:20150428-104629036
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150428-104629036
Official Citation:Magnetic resonance studies of the binding site interactions between phosphorylcholine and specific mouse myeloma immunoglobulin A. M. Goetze and J. H. Richards Biochemistry 1977 16 (2), 228-232 DOI: 10.1021/bi00621a011
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57044
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Apr 2015 18:28
Last Modified:28 Apr 2015 18:28

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