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Physical characterization of a totally synthetic 2[4Fe-4S] clostridial ferredoxin

Smith, Eugene T. and Feinberg, Benjamin A. and Richards, John H. and Tomich, John M. (1991) Physical characterization of a totally synthetic 2[4Fe-4S] clostridial ferredoxin. Journal of the American Chemical Society, 113 (2). pp. 688-689. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20150428-123928699

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Abstract

The 2[4Fe-4S] ferredoxins (Fd) serve as electron carriers in fermentative and photosynthetic pathways. In order to determine the influence of specific amino acids on the electron transferring properties of bacterial ferredoxins, Rabinowitz and co-workers have used semisynthetic procedures to substitute or delete specific amino acids near the N-terminus of native ferredoxins. In this paper we report the first totally synthetic 2[4Fe-4S] ferredoxin, which, along with future synthetic variants, will be used to determine the effects of specific amino acid residues throughout the protein upon both its equilibrium and dynamic properties. The apoprotein was synthesized via standard tBOC procedures on polyvinyl (PAM) resins based on principles outlined by Merrifield.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00002a055DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00002a055PublisherArticle
Additional Information:© 1991 American Chemical Society. Received August 27, 1990. Research at UW-Milwaukee was supported by NIH Grant GM41927 to B.A.F. We thank Dr. M. J. Benecky, Mount Sinai Hospital, Milwaukee, WI, for his assistance in obtaining the CD spectra.
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Funding AgencyGrant Number
NIHGM41927
Issue or Number:2
Record Number:CaltechAUTHORS:20150428-123928699
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150428-123928699
Official Citation:Smith, E. T., Feinberg, B. A., Richards, J. H., & Tomich, J. M. (1991). Physical characterization of a totally synthetic 2[4Fe-4S] clostridial ferredoxin. Journal of the American Chemical Society, 113(2), 688-689. doi: 10.1021/ja00002a055
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57047
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:28 Apr 2015 20:49
Last Modified:03 Oct 2019 08:20

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