Di Bilio, Angel J. and Crane, Brian R. and Wehbi, William A. and Kiser, Cynthia N. and Abu-Omar, Mahdi M. and Carlos, Rose M. and Richards, John H. and Winkler, Jay R. and Gray, Harry B. (2001) Properties of Photogenerated Tryptophan and Tyrosyl Radicals in Structurally Characterized Proteins Containing Rhenium(I) Tricarbonyl Diimines. Journal of the American Chemical Society, 123 (13). pp. 3181-3182. ISSN 0002-7863. doi:10.1021/ja0043183. https://resolver.caltech.edu/CaltechAUTHORS:20150428-143033571
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Abstract
Aromatic amino acid radicals are key intermediates in nucleic acid biosynthesis, DNA repair, dioxygen reduction by cytochrome oxidase, water oxidation by PSII, as well as other biological procesess. In our work on electron tunneling in proteins, we have developed laser flash/quench methods that potentially could facilitate the study of such highly reactive radicals. To test our methods, we are investigating two structurally characterized proteins, [Re(CO)_3(L)(H83)]^+AzM^(2+) and [Re(CO)_3(L)(H107)]^+AzM^(2+) (L ) 1,10-phenanthroline (phen) or 4,7-Me_2phen; Az ) Pseudomonas aeruginosa azurin; M ) Cu or Zn). Of special interest is that calculations and experiments on the H107 protein show that Cu^+ oxidation via electron transfer (ET) through an intervening tyrosine (Cu^+ → Y108^(./) → Re(2+)) is over 2 orders of magnitude faster than optimized (Cu^+ → Re^(2+)) electron tunneling.
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Additional Information: | © 2001 American Chemical Society. Received December 22, 2000. Dedicated to the memory of Jerry Babcock (1946- 2000). Jerry was an enthusiastic colleague and dear friend who helped us in many different ways during the course of this work. He was a master teacher as well as a brilliant scholar, and we will forever be indebted to him for his guidance. Supported by NIH (DK19038 to H.B.G.; GM16424 to J.H.R.). | ||||||||||||
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Issue or Number: | 13 | ||||||||||||
DOI: | 10.1021/ja0043183 | ||||||||||||
Record Number: | CaltechAUTHORS:20150428-143033571 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150428-143033571 | ||||||||||||
Official Citation: | Di Bilio, A. J., Crane, B. R., Wehbi, W. A., Kiser, C. N., Abu-Omar, M. M., Carlos, R. M., . . . Gray, H. B. (2001). Properties of Photogenerated Tryptophan and Tyrosyl Radicals in Structurally Characterized Proteins Containing Rhenium(I) Tricarbonyl Diimines. Journal of the American Chemical Society, 123(13), 3181-3182. doi: 10.1021/ja0043183 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 57058 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Joanne McCole | ||||||||||||
Deposited On: | 29 Apr 2015 20:01 | ||||||||||||
Last Modified: | 10 Nov 2021 21:07 |
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