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Mutations of the Weak Axial Ligand in the Thermus CuA Center Modulates Its Electronic Structure

Slutter, Claire E. and Gromov, Igor and Richards, John H. and Pecht, John and Goldfarb, Daniella (1999) Mutations of the Weak Axial Ligand in the Thermus CuA Center Modulates Its Electronic Structure. Journal of the American Chemical Society, 121 (21). pp. 5077-5078. ISSN 0002-7863. doi:10.1021/ja984361e. https://resolver.caltech.edu/CaltechAUTHORS:20150428-161643812

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Abstract

Here we report the first stable, axial ligand mutations, Met160Gln and Met160Glu, of a Cu_A center in the Thermus thermophilus (TtIICu_A) fragment of cytochrome ba3.2a The binuclear, delocalized (S = 1/2) Cu_A center serves as the initial electron acceptor in cytochrome c oxidases (COX) and nitrous oxide reductase (N_2OR). The Cu_A domains and the type 1 (T1) blue copper centers are members of the cupredoxin superfamily of electron transfer (ET) proteins. The Cu_A centers have two bridging Cys thiolates, two terminal His imidazole ligands, and one weak axial ligand to each copper, a Met thioether or the peptide carbonyl of Glu (Figure 1). The weak axial ligands give each Cu a distorted tetrahedral geometry, similar to the T1 centers.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja984361eDOIArticle
ORCID:
AuthorORCID
Goldfarb, Daniella0000-0001-5714-7159
Additional Information:© 1999 American Chemical Society. Received December 21, 1998. We are grateful to Dr. F. Neese and Prof. P. M. H. Kroneck for providing the EPR simulation program. This work was supported by a National Institutes of Health Grant GM16424 (J.H.R) and the German-Israel Foundation for Scientific Research (D.G. and I.P.). We thank Prof. I. Rubinstein for use of the Jasco V-570 UV/vis/NIR Spectrometer.
Funders:
Funding AgencyGrant Number
NIHGM16424
German-Israel Foundation for Scientific ResearchUNSPECIFIED
Issue or Number:21
DOI:10.1021/ja984361e
Record Number:CaltechAUTHORS:20150428-161643812
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150428-161643812
Official Citation:Mutations of the Weak Axial Ligand in the Thermus CuA Center Modulates Its Electronic Structure Claire E. Slutter, Igor Gromov, John H. Richards, Israel Pecht, and Daniella Goldfarb Journal of the American Chemical Society 1999 121 (21), 5077-5078 DOI: 10.1021/ja984361e
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57074
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:01 May 2015 17:12
Last Modified:10 Nov 2021 21:07

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