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Mechanism of action of coenzyme B_(12). Release of 5′-deoxyinosine on incubation of deoxyinosylcobalamin, 1,2-propanediol and propanediol dehydrase

Jayme, Manfred and Richards, John H. (1971) Mechanism of action of coenzyme B_(12). Release of 5′-deoxyinosine on incubation of deoxyinosylcobalamin, 1,2-propanediol and propanediol dehydrase. Biochemical and Biophysical Research Communications, 43 (6). pp. 1329-1333. ISSN 0006-291X. doi:10.1016/S0006-291X(71)80018-9. https://resolver.caltech.edu/CaltechAUTHORS:20150429-085524057

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Abstract

We wish to report evidence that the covalent bond in coenzyme B_(12) between the cobalt atom and the methylene group (C-5′) of the nucleoside moiety is cleaved during enzymatic conversion of 1,2-propanediol to propionaldehyde. Acquisition of a third hydrogen satisfies the valence at C-5′ created by the cobalt-C-5′ bond cleavage; C-5′ is thus converted to a methyl group. The experimental result to support this assertion is the observation that mixtures of 1,2-propanediol, propanediol dehydrase and deoxyinosylcobalamin liberate 5′-deoxyinosine to solution.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0006-291X(71)80018-9DOIArticle
http://www.sciencedirect.com/science/article/pii/S0006291X71800189PublisherArticle
Additional Information:© 1971 Published by Elsevier Inc. Received 6 May 1971, Available online 6 April 2005. Supported by the National Institutes of Health Grant GMS-10218. We should like to thank the National Institutes of Health for financial support of this work and Professor R. H. Abeles of Brandeis for a generous gift of propanediol dehydrase.
Funders:
Funding AgencyGrant Number
NIHGMS-10218
Other Numbering System:
Other Numbering System NameOther Numbering System ID
Caltech Gates and Crellin Laboratories4229
Issue or Number:6
DOI:10.1016/S0006-291X(71)80018-9
Record Number:CaltechAUTHORS:20150429-085524057
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150429-085524057
Official Citation:Manfred Jayme, John H. Richards, Mechanism of action of coenzyme B12. Release of 5′-deoxyinosine on incubation of deoxyinosylcobalamin, 1,2-propanediol and propanediol dehydrase, Biochemical and Biophysical Research Communications, Volume 43, Issue 6, 18 June 1971, Pages 1329-1333, ISSN 0006-291X, http://dx.doi.org/10.1016/S0006-291X(71)80018-9. (http://www.sciencedirect.com/science/article/pii/S0006291X71800189)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57081
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Apr 2015 16:11
Last Modified:10 Nov 2021 21:07

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