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Secondary isotope effects in dehydration of malic acid by fumarate hydratase

Schmidt, Donald E., Jr. and Nigh, Wesley G. and Tanzer, Christian and Richards, John H. (1969) Secondary isotope effects in dehydration of malic acid by fumarate hydratase. Journal of the American Chemical Society, 91 (21). pp. 5849-5854. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20150429-094618091

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Abstract

The kinetic isotope effects for the dehydration by fumarate hydratase of 2-^3H-1,4-^(14)Cl-/-malic acid and 2,3-^3H-1,4-^(14)C-l-malic acid have been determined and support a carbonium ion mechanism for the enzymatic reaction. The isotope effect on the equilibrium between l-malate and fumarate has also been determined experimentally and agrees closely with that anticipated on the basis of a Bigeleisen treatment.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja01049a026DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja01049a026PublisherArticle
Additional Information:© 1969 American Chemical Society. We should like to thank the National Institutes of Health of the U.S. Public Health Service (Grant GM-10218) for support of this research and Professor J. D. Roberts for a useful suggestion.
Funders:
Funding AgencyGrant Number
NIHGM-10218
Other Numbering System:
Other Numbering System NameOther Numbering System ID
Caltech Gates and Crellin Laboratories3796
Issue or Number:21
Record Number:CaltechAUTHORS:20150429-094618091
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150429-094618091
Official Citation:Secondary isotope effects in dehydration of malic acid by fumarate hydratase Donald E. Schmidt Jr., Wesley G. Nigh, Christian Tanzer, and John H. Richards Journal of the American Chemical Society 1969 91 (21), 5849-5854 DOI: 10.1021/ja01049a026
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57085
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Apr 2015 17:37
Last Modified:03 Oct 2019 08:20

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