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Hydrogen transfer in catalysis by adenosylcobalamin-dependent diol dehydratase

Moore, Kevin W. and Bachovchin, William W. and Gunter, Joel B. and Richards, John H. (1979) Hydrogen transfer in catalysis by adenosylcobalamin-dependent diol dehydratase. Biochemistry, 18 (13). pp. 2776-2782. ISSN 0006-2960. http://resolver.caltech.edu/CaltechAUTHORS:20150429-150734315

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Abstract

Studies [Bachovchin, W. W., et al. (1978) Biochemistry 17, 2218] of the mechanism of inactivation of adenosylcobalamin-dependent diol dehydratase have led to the development of a general method to describe the kinetics of a reaction pathway containing a reservoir of mobile hydrogen. Analysis by this method of catalytic rate measurements for mixtures of 1,2-propanediol and 1, 1-dideuterio-1,2-propanediol supports a mechanism involving an intermediate with three equivalent hydrogens, in which hydrogen transfer from this intermediate to product is the major rate-contributing step. Other results using tritium as a trace label [Essenberg, M. K., et al. (1971) J. Am. Chem. Soc. 93, 1242] are considered in light of these deuterium isotope studies.


Item Type:Article
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http://dx.doi.org/10.1021/bi00580a013 DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi00580a013PublisherArticle
Additional Information:© 1979 American Chemical Society. Received October 2, 1978; revised manuscript received February 13, 1979. This is Contribution No. 5878. This research was supported by National Institutes of Health Grant GM-10218.
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NIHGM-10218
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Caltech Church Laboratory of Chemical Biology5878
Record Number:CaltechAUTHORS:20150429-150734315
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150429-150734315
Official Citation:Hydrogen transfer in catalysis by adenosylcobalamin-dependent diol dehydratase Kevin W. Moore, William W. Bachovchin, Joel B. Gunter, and John H. Richards Biochemistry 1979 18 (13), 2776-2782 DOI: 10.1021/bi00580a013
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57098
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Apr 2015 20:46
Last Modified:30 Apr 2015 20:46

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