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Molecular studies of subspecificity differences among phosphorylcholine-binding mouse myeloma antibodies using ^(31)P nuclear magnetic resonance

Goetze, Andrew M. and Richards, John H. (1978) Molecular studies of subspecificity differences among phosphorylcholine-binding mouse myeloma antibodies using ^(31)P nuclear magnetic resonance. Biochemistry, 17 (9). pp. 1733-1739. ISSN 0006-2960. https://resolver.caltech.edu/CaltechAUTHORS:20150429-153205407

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Abstract

We have determined the pH dependencies of the binding affinities of the mouse myeloma immunoglobulins M603, W3207, and M167 for the haptens phosphorylcholine (PC) and L-α-glycerophosphorylcholine (GPC). These affinities are generally maximal near neutral pH with the exception of the binding of PC by M167 which is strongest at pH 5.5. These data have helped to clarify the nature and relative importance of the ionic interactions between hapten and antibody. ^(31)P nuclear magnetic resonance (NMR) techniques were used to probe the influence of pH on the microenvironment of the phosphate group of several haptens when these were bound to M603, W3207, and M167. The phosphate subsites of M603 and W3207 are both electropositive and also show other similarities; that of M167 has a net electronegative character. The two hydrogen bonds known to be formed between M603 and the phosphate oxygens of PC are also involved in binding GPC and are essentially unaffected by pH in the region 3-9. Studies with the hapten 3-trimethylamino-l-propanol phosphate (TMAPP) show that the binding cavity of M 167 is substantially wider than those of M603 and W3207. These results lead to a detailed molecular model of the pH dependent binding of PC and related haptens to these three antibodies; they further indicate the roles of various amino acid residues in defining the differing ligand specificities of these antibodies.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi00602a023DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi00602a023PublisherArticle
Alternate Title:Molecular studies of subspecificity differences among phosphorylcholine-binding mouse myeloma antibodies using phosphorus-31 nuclear magnetic resonance
Additional Information:© 1978 American Chemical Society. Received August 30, 1977. This work was supported by the President's Fund of the California Institute of Technology and the National Institutes of Health (Grant GM-16424).
Funders:
Funding AgencyGrant Number
Caltech President’s FundUNSPECIFIED
NIHGM-16424
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Other Numbering System NameOther Numbering System ID
Caltech Church Laboratory of Chemical Biology5655
Issue or Number:9
Record Number:CaltechAUTHORS:20150429-153205407
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150429-153205407
Official Citation:Molecular studies of subspecificity differences among phosphorylcholine-binding mouse myeloma antibodies using phosphorus-31 nuclear magnetic resonance Andrew M. Goetze and John H. Richards Biochemistry 1978 17 (9), 1733-1739 DOI: 10.1021/bi00602a023
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57099
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Apr 2015 20:45
Last Modified:03 Oct 2019 08:20

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