Webb, M. Adam and Kiser, Cynthia N. and Richards, John H. and Di Bilio, Angel J. and Gray, Harry B. and Winkler, Jay R. and Loppnow, Glen R. (2000) Resonance Raman Spectroscopy of Met121Glu Azurin. Journal of Physical Chemistry B, 104 (46). pp. 10915-10920. ISSN 1520-6106. https://resolver.caltech.edu/CaltechAUTHORS:20150429-154244398
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Abstract
The resonance Raman spectra for Pseudomonas aeruginosa Met121Glu azurin have been measured at wavelengths throughout the 600-nm absorption band at low and high pH. The spectra of the mutant at pH 3.5 and 7.0 are identical. Analysis of the 600-nm absorption band and resulting resonance Raman excitation profiles under both pH conditions indicates that the excited-state distortions in this mutant are smaller than those in the native protein.
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| Additional Information: | © 2000 American Chemical Society. Received: March 2, 2000; In Final Form: September 11, 2000. Work at the University of Alberta was supported by NSERC; work at Caltech was supported by NIH DK19038. | ||||||||||
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| Issue or Number: | 46 | ||||||||||
| Record Number: | CaltechAUTHORS:20150429-154244398 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150429-154244398 | ||||||||||
| Official Citation: | Resonance Raman Spectroscopy of Met121Glu Azurin M. Adam Webb, Cynthia N. Kiser, John H. Richards, Angel J. Di Bilio, Harry B. Gray, Jay R. Winkler, and Glen R. Loppnow The Journal of Physical Chemistry B 2000 104 (46), 10915-10920 DOI: 10.1021/jp000832j | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 57100 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | Tony Diaz | ||||||||||
| Deposited On: | 30 Apr 2015 20:44 | ||||||||||
| Last Modified: | 03 Oct 2019 08:20 |
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