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Resonance Raman Spectroscopy of Met121Glu Azurin

Webb, M. Adam and Kiser, Cynthia N. and Richards, John H. and Di Bilio, Angel J. and Gray, Harry B. and Winkler, Jay R. and Loppnow, Glen R. (2000) Resonance Raman Spectroscopy of Met121Glu Azurin. Journal of Physical Chemistry B, 104 (46). pp. 10915-10920. ISSN 1520-6106. https://resolver.caltech.edu/CaltechAUTHORS:20150429-154244398

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Abstract

The resonance Raman spectra for Pseudomonas aeruginosa Met121Glu azurin have been measured at wavelengths throughout the 600-nm absorption band at low and high pH. The spectra of the mutant at pH 3.5 and 7.0 are identical. Analysis of the 600-nm absorption band and resulting resonance Raman excitation profiles under both pH conditions indicates that the excited-state distortions in this mutant are smaller than those in the native protein.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/jp000832jDOIArticle
http://pubs.acs.org/doi/abs/10.1021/jp000832jPublisherArticle
ORCID:
AuthorORCID
Webb, M. Adam0000-0001-9160-7251
Kiser, Cynthia N.0000-0002-0102-996X
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2000 American Chemical Society. Received: March 2, 2000; In Final Form: September 11, 2000. Work at the University of Alberta was supported by NSERC; work at Caltech was supported by NIH DK19038.
Funders:
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
NIHDK19038
Issue or Number:46
Record Number:CaltechAUTHORS:20150429-154244398
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150429-154244398
Official Citation:Resonance Raman Spectroscopy of Met121Glu Azurin M. Adam Webb, Cynthia N. Kiser, John H. Richards, Angel J. Di Bilio, Harry B. Gray, Jay R. Winkler, and Glen R. Loppnow The Journal of Physical Chemistry B 2000 104 (46), 10915-10920 DOI: 10.1021/jp000832j
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57100
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Apr 2015 20:44
Last Modified:03 Oct 2019 08:20

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