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The Cu_A Center of a Soluble Domain from Thermus Cytochrome ba_3. An NMR Investigation of the Paramagnetic Protein

Bertini, Ivano and Bren, Kara L. and Clemente, Antonio and Fee, James A. and Gray, Harry B. and Luchinat, Claudio and Malmström, Bo G. and Richards, John H. and Sanders, Donita and Slutter, Claire E. (1996) The Cu_A Center of a Soluble Domain from Thermus Cytochrome ba_3. An NMR Investigation of the Paramagnetic Protein. Journal of the American Chemical Society, 118 (46). pp. 11658-11659. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20150504-120953603

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Abstract

The Cu_A center in subunit II of cytochrome c oxidase, the terminal enzyme of aerobic respiration, transfers electrons from cytochrome c to the proton-pumping machinery in subunit I. The unique electronic absorption and EPR spectra of Cu_A exclude it from classification with the well-studied biological copper centers. High-resolution X-ray structures of Cu_A-containing proteins reveal two copper atoms approximately 2.5 Å apart, bridged by two cysteine sulfurs. Each Cu has a terminal histidine ligand and a weak ligand, methionine for one and a main chain carbonyl for the other. These structures are consistent with earlier EPR measurements and theoretical calculations, which predicted a highly delocalized mixed-valence [Cu(II),Cu(I)] Cu_A site. Here we report ^1H NMR measurements at 600 MHz on a soluble Cu_A domain from Thermus thermophilus cytochrome ba_3.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja9621410DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja9621410PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja9621410PublisherSupporting Information
ORCID:
AuthorORCID
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 1996 American Chemical Society. Received June 25, 1996. Publication Date (Web): November 20, 1996. Support from NIH (J.A.F., GM 35342; H.B.G., DK 19038; J.H.R., GM 16424) is acknowledged. B.G.M. is grateful to the FLORMARPARM Large Scale Facility of the EU, which supported his stay in Florence. The University of Florence is acknowledged for partially supporting C.E.S. and J.H.R. in Florence. Supporting Information Available: A 4 ms 2D NOESY spectrum at pH 4.5 and 278 K (2 pages).
Funders:
Funding AgencyGrant Number
NIHGM 35342
NIHDK 19038
NIHGM 16424
FLORMARPARM Large Scale FacilityUNSPECIFIED
University of FlorenceUNSPECIFIED
Issue or Number:46
Record Number:CaltechAUTHORS:20150504-120953603
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150504-120953603
Official Citation:The CuA Center of a Soluble Domain from Thermus Cytochrome ba3. An NMR Investigation of the Paramagnetic Protein Ivano Bertini, Kara L. Bren, Antonio Clemente, James A. Fee, Harry B. Gray, Claudio Luchinat, Bo G. Malmström, John H. Richards, Donita Sanders, and Claire E. Slutter Journal of the American Chemical Society 1996 118 (46), 11658-11659 DOI: 10.1021/ja9621410
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57197
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:04 May 2015 20:28
Last Modified:03 Oct 2019 08:22

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