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Characterization, Comparative Genomics and Genome Mining for Antibiotics and Secondary Metabolite of two Actinomycetales isolates

Mariita, R. M. and Bhatnagar, S. and Hanselmann, K. and Hossain, M. and Liles, M. and Moss, A. G. and Leadbetter, J. L. and Newman, D. K. (2014) Characterization, Comparative Genomics and Genome Mining for Antibiotics and Secondary Metabolite of two Actinomycetales isolates. In: 2014 ASCB: an International Forum for Cell Biology, December, 2014, Philadelphia, PA. https://resolver.caltech.edu/CaltechAUTHORS:20150504-143203439

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Abstract

Actinomycetes are ubiquitous Gram (+) bacteria commonly found to have high G+C content and best known for their metabolic by-products and novel enzymes [1]. Isolates CCMMD2014 & MRMD2014 were co-cultured from soil impacted by a rusty fire hydrant in Woods Hole, MA. The Streptomyces sp. and Curtobacterium sp. isolates were identified by marker genes for 16S rRNA, rpoB, xylose isomerase, tryptophan synthase beta chain and Cytochrome P450 monooxygenase. Both isolates showed lactic acid fermentation and urease activity. The co-isolates were separated by selective culturing with antibiotics. In addition, whole genome sequencing revealed distinct inherent metabolic pathways in each culture that allowed for mutually exclusive selective culture conditions. Assembly was done using HGAP3 with Celera8 assembler using SMRT portal [2,3]. Annotation was done using the RAST server [4], with 7540 and 3969 CDS for Streptomyces sp. and Curtobacterium sp. respectively being revealed by AMIGene and BASys [5,6]. Subsequently, antiSMASH [7], was used to predict 52 and 26 secondary metabolite biosynthetic clusters that included genes for lantipeptides, terpenes, siderophores, polyketide synthases type I and II, bacteriocin and nonribosomal peptide synthase genes for Streptomyces sp. and Curtobacterium sp. respectively. The isolates have genes of potentially beneficial traits that could help study, among others, the role of fimbrial adhesins and iron in biofilm formation and investigation on natural products.


Item Type:Conference or Workshop Item (Poster)
Related URLs:
URLURL TypeDescription
http://www.ascb.org/files/AllPosterPresentations2014.pdfPublisherArticle
ORCID:
AuthorORCID
Newman, D. K.0000-0003-1647-1918
Additional Information:© 2014 American Society for Cell Biology. TUESDAY-POSTER PRESENTATION. 2014 Microbial Diversity, Marine Biological Laboratory. Pacific Biotechnology. RMM support: AU-CMB Peaks of Excellence summer graduate research award, Selman A. Waksman Endowed Scholarship in Microbial Diversity, Bernard Davis Endowed Scholarship (47802012050), Auburn Graduate School, and the AU-DBS Farrington Fund. We are grateful to Scott Dawson, George OToole, Alison Bulter, Emil Ruff, Arpita Bose and Suzanne Kern for their assistance.
Funders:
Funding AgencyGrant Number
AU-CMB Peaks of Excellence summer graduate research awardUNSPECIFIED
Selman A. Waksman Endowed Scholarship in Microbial DiversityUNSPECIFIED
Auburn Graduate School, Bernard Davis Endowed Scholarship47802012050
AU-DBS Farrington FundUNSPECIFIED
Record Number:CaltechAUTHORS:20150504-143203439
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150504-143203439
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57209
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 May 2015 17:12
Last Modified:03 Oct 2019 08:22

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