Dill, Kilian and Satterlee, James D. and Richards, John H. (1978) ^(13)C nuclear magnetic resonance studies of the binding of isocyanides to various hemoglobins and myoglobins. Biochemistry, 17 (20). pp. 4291-4298. ISSN 0006-2960. doi:10.1021/bi00613a028. https://resolver.caltech.edu/CaltechAUTHORS:20150505-103739032
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Abstract
Interactions between ethyl and isopropyl isocyanides and various hemoglobins and myoglobins have been studied by ^(13)C nuclear magnetic resonance. The results indicate that the chemical shift of the bound isocyanide depends on the structure of the hemoglobin subunit or myoglobin. The resonances exhibited by isocyanides bound to myoglobin are sensitive to pH in contrast to the situation with rabbit and human hemoglobins. β subunits of opossum, rabbit, and human hemoglobins show a significantly greater preferential affinity for CO relative to EIC than do α subunits which have allowed the assignment of resonances. Rabbit, human, and opossum hemoglobin subunits bind ethyl isocyanide without observable preferences and an excess of DPG does not appear to affect this random order of ligation. In contrast, an excess of IHP seems to cause preferential ligation of the α subunits in these hemoglobins. The results have been used to gain insights into the differing characteristics of the ligand binding pockets of these various hemoglobins.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1978 American Chemical Society. Received May 24, 1977; revised manuscript received January 6, 1978. This work was supported by the National Institutes of Health Grants HL 15198, HL 15162, and GM 16424. | |||||||||
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| Issue or Number: | 20 | |||||||||
| DOI: | 10.1021/bi00613a028 | |||||||||
| Record Number: | CaltechAUTHORS:20150505-103739032 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150505-103739032 | |||||||||
| Official Citation: | 13C nuclear magnetic resonance studies of the binding of isocyanides to various hemoglobins and myoglobins Kilian Dill, James D. Satterlee, and John H. Richards Biochemistry 1978 17 (20), 4291-4298 DOI: 10.1021/bi00613a028 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 57227 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 05 May 2015 19:30 | |||||||||
| Last Modified: | 10 Nov 2021 21:09 |
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