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Amphiphilicity is essential for mitochondrial presequence function

Roise, David and Theiler, Franziska and Horvath, Suzanna J. and Tomich, John M. and Richards, John H. and Allison, Daniel S. and Schatz, Gottfried (1988) Amphiphilicity is essential for mitochondrial presequence function. EMBO Journal, 7 (3). pp. 649-653. ISSN 0261-4189.

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We have shown earlier that a mitochondrial presequence peptide can form an amphiphilic helix. However, the importance of amphiphilicity for mitochondrial presequence function became doubtful when an artificial presequence, designed to be non-amphiphilic, proved to be active as a mitochondrial import signal. We now show experimentally that this 'non-amphiphilic' presequence peptide is, in fact, highly amphiphilic as measured by its ability to insert into phospholipid monolayers and to disrupt phospholipid vesicles. This result, and similar tests on three additional artificial presequences (two functionally active and one inactive), revealed that all active presequences were amphiphilic whereas the inactive presequence was non-amphiphilic. One of the active presequence peptides was non-helical in solution and in the presence of detergent micelles. We conclude that amphiphilicity is necessary for mitochondrial presequence function whereas a helical structure may not be essential.

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Additional Information:© 1988 IRL Press Limited. Received on November 4, 1987; Revised on December 23, 1987. We would like to acknowledge gratefully the use of the Microchemical Facility in the laboratory of Professor Leroy E. Hood at Caltech. We would also like to thank Dr E. Lanka for plasmid pJF1 18-EH. This study was supported by grant 3.335-0.86 from the Swiss National Science Foundation, grant CBY-11R01 GM37803-01 from the US Public Health Service and a postdoctoral fellowship (to D.R.) from the Jane Coffin Childs Memorial Fund for Medical Research.
Funding AgencyGrant Number
Swiss National Science Foundation3.335-0.86
U.S. Public Health Service (USPHS)CBY-11R01
U.S. Public Health Service (USPHS)GM37803-01
Jane Coffin Childs Memorial Fund for Medical ResearchUNSPECIFIED
Subject Keywords:amphiphilicity, presequence, mitochondria, protein, import, lipid model systems
Issue or Number:3
Record Number:CaltechAUTHORS:20150505-125902591
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Official Citation:Roise, D., Theiler, F., Horvath, S. J., Tomich, J. M., Richards, J. H., Allison, D. S., & Schatz, G. (1988). Amphiphilicity is essential for mitochondrial presequence function. The EMBO Journal, 7(3), 649-653.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57236
Deposited By: Joanne McCole
Deposited On:06 May 2015 14:38
Last Modified:03 Oct 2019 08:22

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