Eagar, Robert G., Jr. and Bachovchin, William W. and Richards, John H. (1975) Mechanism of action of adenosylcobalamin. 3-Fluoro-1,2-propanediol as substrate for propanediol dehydrase. Mechanistic implications. Biochemistry, 14 (25). pp. 5523-5528. ISSN 0006-2960. https://resolver.caltech.edu/CaltechAUTHORS:20150505-134541144
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20150505-134541144
Abstract
3-Fluoro-1,2-propanediol has been found to be a substrate for propanediol dehydrase and has very similar binding and catalytic constants compared to the natural substrate. The only isolable products of the reaction are acrolein and inorganic fluoride; with 3-fluoro-3,3-dideuterio-l,2-propanediol as substrate, only 3,3-dideuterioacrolein is obtained. These results indicate that the primary product of the reaction is 3-fluoropropionaldehyde which spontaneously loses hydrogen fluoride to yield acrolein. The similar kinetic parameters for the fluorinated as compared to the normal substrate suggest that significant charge does not develop on the fluorinated or, by implication, the natural substrate during any rate-limiting steps of the reaction. These results support a radical, as contrasted to an ionic pathway for reactions involving adenosylcobalamin and diol dehydrase.
| Item Type: | Article | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| |||||||||
| Additional Information: | © 1975 American Chemical Society. Received July 10, 1975. This research was supported by National Institutes of Health Grant GM-10218. | |||||||||
| Funders: |
| |||||||||
| Other Numbering System: |
| |||||||||
| Issue or Number: | 25 | |||||||||
| Record Number: | CaltechAUTHORS:20150505-134541144 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150505-134541144 | |||||||||
| Official Citation: | Mechanism of action of adenosylcobalamin. 3-Fluoro-1,2-propanediol as substrate for propanediol dehydrase. Mechanistic implications Robert G. Eagar Jr., William W. Bachovchin, and John H. Richards Biochemistry 1975 14 (25), 5523-5528 DOI: 10.1021/bi00696a022 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 57239 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 06 May 2015 16:38 | |||||||||
| Last Modified: | 03 Oct 2019 08:22 |
Repository Staff Only: item control page
