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Essential roles of the κ light chain intronic enhancer and 3' enhancer in κ rearrangement and demethylation

Inlay, Matthew and Alt, Frederick W. and Baltimore, David and Xu, Yang (2002) Essential roles of the κ light chain intronic enhancer and 3' enhancer in κ rearrangement and demethylation. Nature Immunology, 3 (5). pp. 463-468. ISSN 1529-2908. doi:10.1038/ni790.

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The κ intronic (MiE_κ) and 3' (3'E_κ) enhancers are both quantitatively important to, but not essential for, immunoglobulin kappa rearrangement. To determine the functional redundancy between these two enhancers, B cells derived from mutant embryonic stem cells—in which both MiE_κ and 3'E_κ were deleted on both kappa alleles—were analyzed for κ rearrangement. Our findings indicate that these double-mutant B cells have essentially no κ rearrangement but do rearrange and express lambda. Therefore, these two kappa enhancers share essential roles in activating V_κJ_κ rearrangement. Our findings also indicate that the two κ enhancers play overlapping and distinct roles in the demethylation of κ in B cells.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 2002 Nature Publishing Group. Received 14 January 2002; Accepted 29 March 2002; Published online: 22 April 2002. We thank M. Schlissel for 3'Eκ^-/- mice and members of Alt laboratory for critical reading of this manuscript. Supported by NIH grant AI44838 (to Y. X.). Competing interests statement: The authors declare that they have no competing financial interests.
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Issue or Number:5
Record Number:CaltechAUTHORS:20150513-151007815
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Official Citation:Inlay, M., Alt, F. W., Baltimore, D., & Xu, Y. (2002). Essential roles of the [kappa] light chain intronic enhancer and 3[prime] enhancer in [kappa] rearrangement and demethylation. [10.1038/ni790]. Nat Immunol, 3(5), 463-468.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57509
Deposited By: Jason Perez
Deposited On:13 May 2015 23:21
Last Modified:10 Nov 2021 21:51

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