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Parallel identification of O-GlcNAc-modified proteins from cell lysates

Tai, Hwan-Ching and Khidekel, Nelly and Ficarro, Scott B. and Peters, Eric C. and Hsieh-Wilson, Linda C. (2004) Parallel identification of O-GlcNAc-modified proteins from cell lysates. Journal of the American Chemical Society, 126 (34). pp. 10500-10501. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20150519-124748642

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Abstract

We report a new strategy for the parallel identification of O-GlcNAc-glycosylated proteins from cell lysates. The approach permits specific proteins of interest to be rapidly interrogated for the modification in any tissue or cell type and can be extended to peptides to facilitate the mapping of glycosylation sites. As an illustration of the approach, we identified four new O-GlcNAc-glycosylated proteins of low cellular abundance (c-Fos, c-Jun, ATF-1, and CBP) and two short regions of glycosylation in the enzyme O-GlcNAc transferase (OGT). The ability to target specific proteins across various tissue or cell types complements emerging proteomic technologies and should advance our understanding of this important posttranslational modification.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja047872b DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja047872bPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja047872bPublisherSupporting Information
ORCID:
AuthorORCID
Hsieh-Wilson, Linda C.0000-0001-5661-1714
Additional Information:© 2004 American Chemical Society. Received April 13, 2004. Publication Date (Web): August 5, 2004. We thank Drs. B. Ramakrishnan and P. Qasba for providing the mutant GalT, Dr. M. Montminy for helpful discussions, Dr. J. Hanover for OGT cDNA, and Dr. P. Snow for protein expression. This research was supported by an NIH training grant (GM07616-21), California TRDRP (13DT-0065), NSF CAREER Award (CHE-0239861), and Alfred P. Sloan Fellowship.
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipGM07616-21
California Tobacco-Related Disease Research Program13DT-0065
NSFCHE-0239861
Alfred P. Sloan FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20150519-124748642
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150519-124748642
Official Citation:Parallel Identification of O-GlcNAc-Modified Proteins from Cell Lysates Hwan-Ching Tai, Nelly Khidekel, Scott B. Ficarro, Eric C. Peters, and Linda C. Hsieh-Wilson Journal of the American Chemical Society 2004 126 (34), 10500-10501 DOI: 10.1021/ja047872b
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57660
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 May 2015 19:54
Last Modified:07 Nov 2017 22:59

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