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Discovery of a TNF-α Antagonist Using Chondroitin Sulfate Microarrays

Tully, Sarah E. and Rawat, Manish and Hsieh-Wilson, Linda C. (2006) Discovery of a TNF-α Antagonist Using Chondroitin Sulfate Microarrays. Journal of the American Chemical Society, 128 (24). pp. 7740-7741. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20150519-141749917

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Abstract

We report the first example of synthetic chondroitin sulfate (CS) microarrays to rapidly identify glycosaminoglycan−protein interactions and probe the specificity of proteins for distinct sulfation sequences. Using the microarrays, we identify a novel interaction between CS and TNF-α, a proinflammatory cytokine involved in rheumatoid arthritis, Crohn's disease, and psoriasis. Moreover, we demonstrate that CS-E tetrasaccharides and polysaccharides enriched in the CS-E sulfation motif can inhibit the activity of this therapeutically important cytokine. We anticipate that carbohydrate microarrays will accelerate our understanding of glycosaminoglycan−protein interactions and the role of sulfation in modulating physiological and disease states.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja061906t DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja061906tPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja061906tPublisherSupporting Information
ORCID:
AuthorORCID
Hsieh-Wilson, Linda C.0000-0001-5661-1714
Additional Information:© 2006 American Chemical Society. Received March 20, 2006. Publication Date (Web): May 27, 2006. We thank Dr. J. L. Riechmann, Director of the Millard and Muriel Jacobs Genetics and Genomics Laboratory at Caltech for assistance with printing the microarrays, and Dr. Susan Ou and Claude J. Rogers. This work was supported by the HFSP, NIH (RO1 NS045061, L.H.W.), NSF (S.E.T.), and HHMI.
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
NIHRO1 NS045061
NSFUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:24
Record Number:CaltechAUTHORS:20150519-141749917
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150519-141749917
Official Citation:Discovery of a TNF-α Antagonist Using Chondroitin Sulfate Microarrays Sarah E. Tully, Manish Rawat, and Linda C. Hsieh-Wilson Journal of the American Chemical Society 2006 128 (24), 7740-7741 DOI: 10.1021/ja061906t
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:57669
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 May 2015 21:29
Last Modified:03 Oct 2019 08:27

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